NK cell receptor gene of the KIR family with two IG domains but highest homology to KIR receptors with three IG domains.
NK cell receptor gene of the KIR family with two IG domains but highest homology to KIR receptors with three IG domains.
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DOI：
10.1111/j.1399-0039.1996.tb02647.x
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发表时间：
1996-10
期刊：
影响因子：
--
通讯作者：
A. Selvakumar;U. Steffens;Bo Dupont
中科院分区：
文献类型：
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作者：
A. Selvakumar;U. Steffens;Bo Dupont
杀伤细胞抑制性受体（KIR）是由自然杀伤（NK）细胞和一些T细胞表达的表面糖蛋白。它们识别多态性人类HLA I类分子。两个KIR家族已被鉴定并命名为p58和p70。p58基因家族编码具有两个细胞外免疫球蛋白（IG）结构域的I型膜蛋白，而p70基因具有三个IG结构域。我们在这里报告了一个新的KIR cDNA的克隆和表征，从肿瘤细胞系与NK反应（YT和NK-92）。该基因也在正常细胞系NK 3.3和从一些但不是所有正常供体获得的NK细胞中表达。克隆KIR 103 AS具有与KIR一致的开放阅读框架，其具有两个胞外IG结构域、跨膜区和含有单个src同源2（SH 2）结合基序的114个氨基酸长的胞质结构域。KIR 103 AS的膜远端IG结构域与p70 KIR的第一个IG结构域具有最高的同源性，并且与p58 KIR的第一个IG结构域显著不同。KIR 103 AS的第二个近膜IG结构域与p70和p58 KIR的近膜IG结构域具有相似且高度的同源性。因此，KIR 103 AS的胞外结构域与p70和p58基因共享特征：结构域结构与p58 KIR相同，但序列同源性与p70 KIR密切匹配。推定的跨膜和胞质结构域明显不同于所有以前报道的KIR cDNA。
The killer cell inhibitory receptors (KIRs) are surface glycoproteins expressed by natural killer (NK) cells and some T cells. They recognize polymorphic human HLA class I molecules. Two families of KIRs have been identified and named p58 and p70. The p58 family of genes encode type I membrane proteins with two extracellular immunoglobulin (Ig) domains, while the p70 genes have three Ig domains. We here report the cloning and characterization of a novel KIR cDNA obtained from tumor cell lines with NK reactivity (YT and NK-92). This gene is also expressed in the normal cell line NK 3.3 and in NK cells obtained from some but not all normal donors. The clone, KIR103AS, has an open reading frame consistent with a KIR with two extracellular Ig domains, a transmembrane region and a 114 amino acid long cytoplasmic domain containing a single src homology 2 (SH2) binding motif. The membrane distal Ig domain of KIR103AS has highest homology with the first Ig domain of p70 KIRs and differs significantly from the first Ig domain of p58 KIRs. The second, membrane proximal Ig domain of KIR103AS has similar and high homology with the membrane proximal Ig domains of both p70 and p58 KIRs. The extracellular domains of KIR103AS therefore share characteristic features with both p70 and p58 genes: the domain structure is identical to p58 KIRs but the sequence homology matches closely with p70 KIRs. The putative transmembrane and cytoplasmic domains are distinctly different from all previously reported KIR cDNAs.