Host recognition by a polyphagous lepidopteran (Helicoverpa armigera):: primary host plants, host produced volatiles and neurosensory stimulation
Host recognition by a polyphagous lepidopteran (Helicoverpa armigera):: primary host plants, host produced volatiles and neurosensory stimulation
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DOI：
10.1111/j.1365-3032.2006.00517.x
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发表时间：
2006-09-01
影响因子：
1.5
通讯作者：
Cribb, B. W.
中科院分区：
文献类型：
--
作者：
Rajapakse, C. N. K.;Walter, G. H.;Cribb, B. W.
在多食性昆虫寻找寄主的行为中，一个重要的问题是，昆虫是否能识别许多植物共同使用的一套化学物质或模板？为了回答这个问题，我们用气相色谱法（GC）对棉铃虫（鳞翅目：夜蛾科）常用的寄主植物（木豆、烟草、棉花和豆类）和非寄主植物（绵草和夹竹桃）的顶空挥发物进行了筛选。在本研究中，我们假设木豆是昆虫的主要寄主植物，以比较不同试验植物的EAG反应。并比较了不同生理状态雌鸽（未交配雌鸽和已交配雌鸽）对鸽豆挥发物的eeg反应。采用气相色谱-质谱联用技术（GC- ms）鉴定了鸽豆顶空气中相对高浓度的8种电生理活性化合物。这些成分包括三种绿叶挥发物[(2E)-己烯醛，(3Z)-己烯乙酸酯和(3Z)-己烯基-2-甲基丁酸盐]和五种单萜烯（-蒎烯，-月桂烯，柠檬烯，e - -辛烯和芳樟醇）。其他被测试的寄主植物含有这些电生理活性化合物的一小部分，甚至非寄主植物也含有这些化合物的一部分，它们的浓度都比鸽豆低。蛾的生理状态或性别对这些化合物的反应没有影响。目前的研究表明，一些寄主植物可以成为飞蛾寻找寄主的主要目标，而其他寄主植物是偶然的或次要的目标。
An important question in the host-finding behaviour of a polyphagous insect is whether the insect recognizes a suite or template of chemicals that are common to many plants? To answer this question, headspace volatiles of a subset of commonly used host plants (pigeon pea, tobacco, cotton and bean) and nonhost plants (lantana and oleander) of Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) are screened by gas chromatography (GC) linked to a mated female H. armigera electroantennograph (EAG). In the present study, pigeon pea is postulated to be a primary host plant of the insect, for comparison of the EAG responses across the test plants. EAG responses for pigeon pea volatiles are also compared between females of different physiological status (virgin and mated females) and the sexes. Eight electrophysiologically active compounds in pigeon pea headspace are identified in relatively high concentrations using GC linked to mass spectrometry (GC-MS). These comprised three green leaf volatiles [(2E)-hexenal, (3Z)-hexenylacetate and (3Z)-hexenyl-2-methylbutyrate] and five monoterpenes (alpha-pinene,beta-myrcene, limonene, E-beta-ocimene and linalool). Other tested host plants have a smaller subset of these electrophysiologically active compounds and even the nonhost plants contain some of these compounds, all at relatively lower concentrations than pigeon pea. The physiological status or sex of the moths has no effect on the responses for these identified compounds. The present study demonstrates how some host plants can be primary targets for moths that are searching for hosts whereas the other host plants are incidental or secondary targets.