Aptamer selection for the inhibition of cell adhesion with fibronectin as target
Aptamer selection for the inhibition of cell adhesion with fibronectin as target
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DOI：
10.1016/j.bmcl.2004.05.042
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发表时间：
2004-08-02
影响因子：
2.7
通讯作者：
Aoyama, Y
中科院分区：
文献类型：
--
作者：
Ogawa, A;Tomita, N;Aoyama, Y
使用免疫化十肽H2 N-RGDSPASSKP-CO2 H的亲和柱从含有随机40聚体序列的86聚体单链寡脱氧核苷酸（ODN）池中选择RGD结合适体。由此获得的富集文库针对吸附的纤连蛋白进一步选择，并且在大肠杆菌中单克隆化各个适体并测序以得到一对高度同源的ODN，其确实抑制纤连蛋白-整联蛋白介导的细胞粘附。(C)2004爱思唯尔有限公司版权所有。
An affinity column immunobilizing a decapeptide H2N-RGDSPASSKP-CO2H was used to select RGD-binding aptamers from a pool of 86-mer single-strand oligodeoxynucleotides (ODNs) containing a random 40-mer sequence. The enriched library thus obtained was further selected against adsorbed fibronectin and individual aptamers were monocloned in E coli and sequenced to give a couple of highly homologous ODNs, which indeed inhibited fibronectin-integrin mediated cell adhesion. (C) 2004 Elsevier Ltd. All right, reserved.