P53 GENE-MUTATIONS IN GASTRIC AND ESOPHAGEAL CANCERS
P53 GENE-MUTATIONS IN GASTRIC AND ESOPHAGEAL CANCERS
复制标题
DOI：
10.1016/0016-5085(92)90022-q
复制
发表时间：
1992-09-01
期刊：
影响因子：
29.4
通讯作者：
ISONO, K
中科院分区：
文献类型：
--
作者：
IMAZEKI, F;OMATA, M;ISONO, K
采用聚合酶链反应和直接核苷酸测序的方法，在10例胃腺癌和5例食管鳞状细胞癌中检测了p53基因5、6、7、8外显子点突变的存在。在5例胃癌和4例食管癌中发现p53基因突变。胃癌的突变包括4个缺失突变（外显子5和8）和1个框架移位（外显子7）。在食管癌中，发现了3个缺失突变（6、7和8外显子）和1个内含子剪接供体位点的点突变。在这两种癌症的7个缺失突变中，5个显示从G到A的转变，2个显示从G到t的转变。所有这些变化都发生在p53蛋白的高度保守区域。这些结果提示p53基因突变是胃腺癌和食管鳞状细胞癌发病机制中的遗传事件。
The presence of point mutation of the p53 gene in exons 5, 6, 7, and 8 was examined in 10 cases of gastric adenocarcinoma and 5 cases of esophageal squamous cell carcinoma by polymerase chain reaction and direct nucleotide sequencing. Mutations of the p53 gene were found in 5 cases of gastric cancer and 4 cases of esophageal cancer. The mutations in the stomach cancers consisted of four missence mutations (exons 5 and 8) and one frame shift (exon 7). In the esophageal cancers, three missence mutations (exons 6, 7, and 8) and one point mutation within the splice donor site of intron 5 were found. Of the seven missence mutations in the two cancers, five showed the transition from G to A and two from G to T. All these changes occurred in the highly conserved region of the p53 protein. These results suggest that mutations of the p53 gene are genetic events in the pathogenesis of gastric adenocarcinoma and esophageal squamous cell carcinoma.