C/EBP homologous protein induces mesangial cell apoptosis under hyperglycemia
C/EBP homologous protein induces mesangial cell apoptosis under hyperglycemia
复制标题
高血糖下C/EBP同源蛋白诱导系膜细胞凋亡
DOI：
10.3892/mmr.2012.1234
复制
发表时间：
2013-02-01
影响因子：
3.4
通讯作者：
Zhang, Xiaoming
中科院分区：
文献类型：
--
作者：
He, Daqiang;Li, Jianqiong;Zhang, Xiaoming
众所周知，糖尿病会导致肾脏损害；然而，其机制仍然难以捉摸。本研究旨在探讨C/EBP同源蛋白（CHOP）在高血糖中内质网应激介导的系膜细胞凋亡中的重要作用。在正常（对照组）和高糖（高糖组）培养基中培养系膜细胞。TUNEL染色观察各组细胞凋亡情况。免疫组织化学和western blot检测CHOP和caspase-3的表达。在高糖培养基中培养24 h后，tunel阳性细胞显著增加(P
Diabetes mellitus is known to cause kidney impairment; however, the mechanism remains elusive. The aim of this study was to investigate the role of C/EBP homologous protein (CHOP), an important protein in endoplasmic reticulum stress-mediated mesangial cell apoptosis in hyperglycemia. Mesangial cells were cultured in normal (control group) and high glucose medium (high glucose group). TUNEL staining was performed to assess apoptotic cells in the groups. The expression of CHOP and caspase-3 was also assayed by immunohistochemistry and western blot analysis. Following 24 h culture in high glucose medium, TUNEL-positive cells were observed to be significantly increased (P