Assessing genetic structure, diversity of bacterial aerosol from aeration system in an oxidation ditch wastewater treatment plant by culture methods and bio-molecular tools
Assessing genetic structure, diversity of bacterial aerosol from aeration system in an oxidation ditch wastewater treatment plant by culture methods and bio-molecular tools
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利用培养方法和生物分子工具评估氧化沟污水处理厂曝气系统细菌气溶胶的遗传结构和多样性
DOI：
10.1007/s10661-012-2578-0
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发表时间：
2013-01-01
影响因子：
3
通讯作者：
Liu, Junxin
中科院分区：
文献类型：
--
作者：
Li, Lin;Han, Yunping;Liu, Junxin
采用旋转曝气刷对北京某城市污水处理厂氧化沟空气细菌排放进行了研究。在3个月（4、5、6月）的时间里，在离旋转刷的不同距离、离水面的不同高度和不同运行状态下采集微生物样本，以估计微生物的季节变化和与站点相关的分布特征。利用培养法分析细菌气溶胶的浓度，利用生物分子技术分析其优势种、遗传结构和多样性。结果表明：6月份总微生物浓度最高，4月份最低；机械旋转使空气中可培养细菌的浓度和多样性在旋转刷前后发生显著变化。旋转刷附近浓度最高（931±129 ~ 3952±730 CFU/m3），浓度随距离和高度的增加而降低。细菌群落聚合酶链反应和变性梯度凝胶电泳结果表明，随着水面高度的增加，多样性逐渐下降，而在相同高度时多样性保持相对稳定。DNA序列分析鉴定的优势菌属厚壁菌门。从生物气溶胶中分离出非液化莫拉菌和臭黄杆菌等致病性菌种。由于涉及严重的健康风险，应监测和控制污水处理工人接触由刷式曝气器引起的空气中微生物。
Airborne bacteria emissions from oxidation ditch with rotating aeration brushes were investigated in a municipal wastewater treatment plant in Beijing, China. Microbial samples were collected at different distances from the rotating brushes, different heights above the water surface, and different operation state over a 3-month period (April, May, and June) in order to estimate the seasonal variation and site-related distribution characteristics of the microorganisms present. The concentration of bacterial aerosol was analyzed by culture methods, while their dominant species, genetic structure and diversity were assayed using bio-molecular tools. Results showed that total microbial concentrations were highest in June and lowest in April. The mechanical rotation caused remarkable variation in concentration and diversity of culturable airborne bacteria before and after the rotating brushes. The highest concentration was observed near the rotating brushes (931 ± 129–3,952 ± 730 CFU/m3), with concentration decreasing as distance and height increased. Bacterial community polymerase chain reaction and denaturing gradient gel electrophoresis indicated that diversity decreased gradually with increasing height above the water surface but remained relatively constant at the same height. All dominant bacteria identified by DNA sequence analysis belonged toFirmicutes. Pathogenic species such asMoraxella nonliquefaciensandFlavobacterium odoratumwere isolated from the bioaerosols. Due to the serious health risks involved, exposure of sewage workers to airborne microorganisms caused by brush aerators should be monitored and controlled.