Analysis of the expression of heme oxygenase-1 gene in human alveolar epithelial cells exposed to cigarette smoke condensate
Analysis of the expression of heme oxygenase-1 gene in human alveolar epithelial cells exposed to cigarette smoke condensate
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DOI：
10.2131/jts.31.99
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发表时间：
2006-05-01
影响因子：
2
通讯作者：
Yoshida, Takemi
中科院分区：
文献类型：
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作者：
Fukano, Yasuo;Oishi, Masako;Yoshida, Takemi
气道上皮暴露于吸入的外源。吸烟引起的肺泡上皮损伤被认为是吸烟相关肺部疾病发病机制中的一个重要过程。目前测量香烟烟雾毒性的机制测定主要集中在致癌作用上。然而，需要设计与其他疾病过程相关的测定。氧化应激与包括慢性阻塞性肺病在内的许多呼吸系统疾病的发病机制有关。因此，我们评估了吸烟的体外研究是否适合检查 HO-I mRNA 表达。将人肺上皮细胞系 A549 暴露于香烟烟雾的颗粒部分（香烟烟雾冷凝物；CSC）并检查 HO-I mRNA 的诱导情况。 CSC 的 HO-I 基因表达呈剂量依赖性增加。与由烤烟或白肋烟制备的CSC对HO-1 mRNA的诱导相比，来自烤烟的CSC似乎倾向于比来自白肋烟的CSC诱导更高的HO-1 mRNA。本研究可以举例说明 HO-1 mRNA 表达测定作为香烟烟雾诱导应激的生物学相关指标，其中源自香烟烟雾的 CSC 与 HO-1 表达的增加呈正相关，并且可以检测烟草类型的差异。
Airway epithelium is exposed to inhaled exogenous sources. Injury of the alveolar epithelium by cigarette smoking is presumed to be an important process in the pathogenesis of smoking-related pulmonary diseases. Current mechanistic assays that measure the toxicity of cigarette smoke focus on carcinogenesis. However, there is a need to design assays relevant to other disease processes. Oxidative stress is implicated in the pathogenesis of many respiratory diseases including chronic obstructive pulmonary disease. Therefore, we evaluated whether in vitro studies of cigarette smoking are appropriate to examine HO-I mRNA expression. The human lung epithelial cell line A549 was exposed to the particulate fraction of cigarette smoke (Cigarette Smoke Condensate; CSC) and examined for the induction of HO-I mRNA. HO-I gene expression by CSC is increased dose-dependently. In comparison of the induction of HO-1 mRNA by CSC prepared from flue-cured or Burley tobacco, CSC from flue-cured tobacco seems to tend to induce an mRNA of HO-I higher than CSC from Burley tobacco. The adaptation of HO-1 mRNA expression assay as a biologically relevant indicator of cigarette smoke-induced stress may be exemplified in this study whereby CSC derived from cigarette smoke positively correlated with an increase in HO-1 expression and the difference of the type of tobacco can be detected.