Direct detection of antibiotic resistance genes in specimens of chicken and pork meat
Direct detection of antibiotic resistance genes in specimens of chicken and pork meat
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DOI：
10.1016/j.ijfoodmicro.2006.07.015
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发表时间：
2007-01-01
影响因子：
5.4
通讯作者：
Biavasco, Francesca
中科院分区：
文献类型：
--
作者：
Garofalo, Cristiana;Vignaroli, Carla;Biavasco, Francesca
细菌中的抗生素耐药性（AR）是对人类健康的主要威胁，在过去几十年中由于抗生素在医学、农业和兽医实践中的广泛使用以及作为畜牧业中的生长促进剂而施加的选择性压力而出现。11个基因[泰特（M）、泰特（O）、泰特（K）、tetA（A）、tetB（B）、tetC（C）、vanA、vanB、aac（6 '）-Ie a-A（2”）-Ia、mecA、blaZ]采用基于PCR的分子生物学方法，分析了生猪肉、鸡肉、发酵香肠以及相关家畜粪便样品中对临床上广泛使用的一些抗生素的编码耐药性扩增直接从标本中提取的细菌DNA。在11种AR基因中，有一些非常普遍，在鸡肉和猪粪便中检测到的数量最多。在肉类中发现最多的基因是泰特（K）和vanB（B）; vanB和mecA最少。所有11个决定因素，检测粪便样品中除了mecA，这是只发现在鸡粪便。在所有粪便样品中均检测到β-内酰胺酶（B）和β-内酰胺酶（C）。AR基因的频率是没有明显不同的肉类相比，粪便标本的相关动物，除了vanB，这是更普遍的粪便。我们的研究结果表明，AR基因在食品相关细菌中非常普遍，AR污染可能与育种而不是加工技术有关。最后，这项工作中使用的不依赖于培养的分子方法被证明是一种快速可靠的替代传统工具，以确定食品中AR基因的流行。(c)2006 Elsevier B. V.保留所有权利。
Antibiotic resistance (AR) in bacteria, a major threat to human health, has emerged in the last few decades as a consequence of the selective pressure exerted by the widespread use of antibiotics in medicine, agriculture and veterinary practice and as growth promoters in animal husbandry. The frequency of 11 genes [tet(M), tet(O), tet(K), erm(A), erm(B), erm(C), vanA, vanB, aac (6')-Ie a-A (2")-Ia, mecA, blaZ] encoding resistance to some antibiotics widely used in clinical practice was analysed in raw pork and chicken meat and in fermented sausages as well as in faecal samples from the relevant farm animals using a molecular approach based on PCR amplification of bacterial DNA directly extracted from specimens. Some of the 11 AR genes were highly prevalent, the largest number being detected in chicken meat and pig faeces. The genes found most frequently in meat were tet(K) and erm(B); vanB and mecA were the least represented. All 11 determinants were detected in faecal samples except mecA, which was found only in chicken faeces. erm(B) and erm(C) were detected in all faecal samples. The frequency of AR genes was not appreciably different in meat compared to faecal specimens of the relevant animal except for vanB, which was more prevalent in faeces. Our findings suggest that AR genes are highly prevalent in food-associated bacteria and that AR contamination is likely related to breeding rather than processing techniques. Finally, the cultivation-independent molecular method used in this work to determine the prevalence of AR genes in foods proved to be a rapid and reliable alternative to traditional tools. (c) 2006 Elsevier B.V. All rights reserved.