The Drosophila TGF-alpha-like protein Gurken: expression and cellular localization during Drosophila oogenesis.
The Drosophila TGF-alpha-like protein Gurken: expression and cellular localization during Drosophila oogenesis.
复制标题
果蝇 TGF-α 样蛋白 Gurken：果蝇卵子发生过程中的表达和细胞定位。
DOI：
10.1016/0925-4773(96)00567-9
复制
发表时间：
1996
影响因子：
2.6
通讯作者：
Schüpbach,T
中科院分区：
文献类型：
--
作者：
Neuman-Silberberg,FS;Schüpbach,T
果蝇卵和胚胎前后和背腹极性的建立取决于基因 gurken、cornichon 和 Egfr（果蝇表皮生长因子受体同源物）的功能。这些基因编码在生殖系细胞和卵巢体细胞卵泡细胞之间传递信息的信号转导途径的成分，gurken 编码转化生长因子-α 样蛋白，并且是卵泡细胞上存在的 Egfr 的假定生殖系配体。在卵子发生中期，格肯转录物在空间上定位于卵母细胞未来的背前皮质。为了分析 Gurken 蛋白的分布模式，我们制备了针对 Gurken 的抗体。我们在这里描述了 Gurken 蛋白在野生型卵巢和许多背腹模式突变体卵巢中的分布模式。通过免疫印迹，我们检测到 Gurken 蛋白的一种主要形式，它可能对应于未加工的蛋白质。
The establishment of anterior-posterior and dorsal-ventral polarity of the Drosophila egg and embryo depends on the function of the genes gurken, cornichon and Egfr (Drosophila epidermal growth factor receptor homolog). These genes encode components of a signal transduction pathway that transmits information between the germline cells and the somatic follicle cells of the ovary, gurken encodes a transforming growth factor-α-like protein and is a putative germline ligand of the Egfr present on the follicle cells. In mid-oogenesis the gurken transcript becomes spatially localized to the future dorsal-anterior cortex of the oocyte. To analyze the distribution pattern of Gurken protein we prepared antibodies against Gurken. We describe here the distribution pattern of the Gurken protein in wild-type ovaries and in ovaries from a number of dorsal-ventral patterning mutants. By immunoblotting we detect one major form of the Gurken protein, which likely corresponds to the unprocessed protein.