Pre-treatment and utilization of raw glycerol from sunflower oil biodiesel for growth and 1,3-propanediol production by Clostridium butyricum
Pre-treatment and utilization of raw glycerol from sunflower oil biodiesel for growth and 1,3-propanediol production by Clostridium butyricum
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DOI：
10.1002/jctb.1917
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发表时间：
2008-07-01
影响因子：
3.4
通讯作者：
Matsumura, Masatoshi
中科院分区：
文献类型：
--
作者：
Asad-ur-Rehman;Saman, Wijesekara R. G.;Matsumura, Masatoshi
背景技术背景：研究了向日葵籽油生物柴油粗甘油（SOB-RG）的预处理工艺及其发酵生产1，3-丙二醇的工艺条件。（pH 4.0）和B级（pH 5.0）磷酸处理的SOB-RG与20 g甘油L-1的纯甘油的那些相似;即，18.5+/- 0.707%至20.5 +/- 0.7%抑制。在A级中，通过用正己醇洗涤A级粗甘油两次（A-2级），在40 g甘油L-1下，生长抑制从85.25 +/- 0.35%降低至32 +/- 1.4%（降低53.25%）。当甘油浓度为20 g/L时，厌氧分批培养的产物生成和底物消耗的动力学参数几乎相同，而当甘油浓度为50 g/L时，体积生产率（Q（p））和1，3-丙二醇生成比速率（q（p））分别从1.13 g/L提高到1.85 g/L h（-1）和1.60 g/g提高到2.65 g/g h（-1）。结论：对SOB-RG进行预处理是开发利用SOB-RG生产1，3-丙二醇的生物工艺技术的必要条件。(C)2008化学工业协会。
BACKGROUND: The objective of the present work is to report an efficient pre-treatment process for sunflower oil biodiesel raw glycerol (SOB-RG) and its fermentation to 1,3-propanediol.RESULTS: The growth inhibition percentages of Clostridium butyricum DSM 5431 on grade A (pH 4.0) and grade B (pH 5.0) phosphoric acid-treated SOB-RG were similar to those of pure glycerol at 20 g glycerol L-1; i.e., 18.5 +/- 0.707% to 20.5 +/- 0.7% inhibition. In grade A, growth inhibition was reduced from 85.25 +/- 0.35% to 32 +/- 1.4% (a 53.25% reduction) at 40 g glycerol L-1 by washing grade A raw glycerol twice with n-hexanol (grade A-2). The kinetic parameters for product formation and substrate consumption in anaerobic batch cultures gave almost similar values at 20 g glycerol L-1, while at 50 g glycerol L-1 volumetric productivity (Q(p)) and specific rate of 1,3-propanediol formation (q(p)) were improved from 1.13 to 1.85 g L-1 h(-1) and 1.60 to 2.65 g g(-1) h(-1), respectively, by employing grade A-2 raw glycerol, while the yields were similar (0.5-0.52 g g(-1)).CONCLUSION: The results are important as the pre-treatment of SOB-RG is necessary to develop bioprocess technologies for conversion of SOB-RG to 1,3-propanediol. (C) 2008 Society of Chemical Industry.