Chronic Vascular Pulmonary Dysplasia Associated with Neonatal Hyperoxia Exposure in the Rat
Chronic Vascular Pulmonary Dysplasia Associated with Neonatal Hyperoxia Exposure in the Rat
复制标题
大鼠慢性血管性肺发育不良与新生儿高氧暴露相关
DOI：
10.1203/00006450-198701000-00005
复制
发表时间：
1987
影响因子：
3.6
通讯作者：
D. Thibeault
中科院分区：
文献类型：
--
作者：
S. Shaffer;D. O'Neill;S. K. Bradt;D. Thibeault
摘要：在出生后前8天暴露于0.96-1.0 FiO 2的雄性大鼠中研究了新生儿高氧的慢性后遗症。在58日龄时，将心肺功能和形态学变化与对照组进行比较。在麻醉下持续测量右心室收缩压，O2组右心室收缩压升高（29.5 mm Hg ± 3.1 vs 23.2 mm Hg ± 3.5，p< 0.001）。两组之间的肺和心脏重量相似。然而，O2组的右心室重量增加（0.197 g ± 0.023 vs 0.175 g ± 0.020，p< 0.001）。空气压力-容积曲线相似，但在O2大鼠中，盐水放气曲线左移，最大液体肺容量更大（14.1 ± 1.2 vs 12.0 ± 0.7 ml，p<0.001）。在100 cm H2O下用钡-凝胶混合物灌注肺动脉，并在25 cm H2O下用福尔马林固定肺。肺镜检查发现肺泡结构的不典型改变，包括不规则的肺泡扩大和不完全的肺泡分隔。肺的形态测量研究表明，O2大鼠的肺实质体积比例增加（0.865 ± 0.020 vs 0.850 ± 0.019，p<0.05），平均线性截距增加（72.3 μ m ± 9.5 vs 53.6 μ m ± 5.0，p<0.001），每平方毫米肺泡数减少（207 ± 34对319 ± 39，p<0.001）和更少的小动脉（20-200 nm）/mm 2（8.7 ±1.3对14.9 ± 2.4，p<0.001）。每100个肺泡的小动脉数量相似。我们的结论是，有限的和具体的新生儿高氧与慢性形态和功能的心肺变化在成年大鼠。这些变化包括小肺动脉数量减少、右心室压力升高和肺气肿样气隙变化。
ABSTRACT: Chronic sequelae of neonatal hyperoxia was studied in male rats exposed to 0.96-1.0 FiO2 for the first 8 days of life. At 58 days of age functional and morphologic cardiopulmonary changes were compared with controls. Right ventricular systolic pressure was measured percutaneously under anesthesia and was increased in the O2 group (29.5 mm Hg ± 3.1 versus 23.2 mm Hg ± 3.5, p< 0.001). Lung and heart weights were similar between groups. Right ventricular weights however were increased in the O2 group (0.197 g ± 0.023 versus 0.175 g ± 0.020, p< 0.001). Air pressure-volume curves were similar but in the O2 rats saline deflation curves were shifted left and maximal fluid lung volumes were greater (14.1 ± 1.2 versus 12.0 ± 0.7 ml, p<0.001). Pulmonary arteries were perfused at 100 cm H2O with a barium-gel mixture and lungs were fixed at 25 cm H2O with formalin. Microscopic examination of lungs revealed dysplastic changes of alveolar architecture which included irregularly enlarged alveoli and incomplete alveolar septation. Morphometric studies of the lungs showed that the O2 rats had an increased volume proportion of parenchyma (0.865 ± 0.020 versus 0.850 ± 0.019, p<0.05), increased mean linear intercept (72.3 urn ± 9.5 versus 53.6/tin ± 5.0, p<0.001), decreased number of alveoli per mm2 (207 ± 34 versus 319 ± 39, p<0.001) and fewer small arteries (20-200 nm) per mm2 (8.7 ±1.3 versus 14.9 ± 2.4, p<0.001). The number of small arteries/100 alveoli was similar. We conclude that limited and specifically timed neonatal hyperoxia is associated with chronic morphologic and functional cardiopulmonary changes in the adult rat. These changes include a decrease in the number of small pulmonary arteries, elevated right ventricular pressure, and emphysema-like changes in the air spaces.
新生仔猪因氧气引起的肺损伤。
影响因子：
2.5
作者：
Yam,J;Roberts,RJ
通讯作者：
Roberts,RJ