Fine mapping of BH1, a gene controlling lemma and palea development in rice
Fine mapping of BH1, a gene controlling lemma and palea development in rice
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DOI：
10.1007/s00299-013-1457-7
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发表时间：
2013-05
影响因子：
6.2
通讯作者：
Xiangjin Wei;Xuanwen Zhang;G. Shao;Jiwai He;G. Jiao;Li-hong Xie;Z. Sheng;Shaoqing Tang;P. Hu-P.-H
中科院分区：
文献类型：
--
作者：
Xiangjin Wei;Xuanwen Zhang;G. Shao;Jiwai He;G. Jiao;Li-hong Xie;Z. Sheng;Shaoqing Tang;P. Hu-P.-H
关键信息一个新的水稻花器官突变体bh 1，对产量有负效应。bh 1缺失了一个55 kb的染色体片段。从水稻品种Asominori的T-DNA文库中筛选出的花突变体dbh 1，其外稃和内稃在花发育后期才出现异常的曲线状外观，最终形成喙状船体。突变体对小麦的千粒重、结实率和发芽率有负效应。遗传分析表明，该突变体的表型由一对隐性基因控制。通过图位分析，最终将BH 1基因定位于2号染色体长臂上约87. 5 kbp的区域。对该区域基因内容的分析表明，突变涉及55 kbp延伸的丢失，包含四个开放阅读框架。qRT-PCR分析表明，与外稃和内稃发育及身份鉴定相关的基因OsMADS 1、OsMADS 14、OsMADS 15、OsMADS 18、REP 1、CFO 1和DL在bh 1突变体中表达下调，推测bh 1是一个重要的花器官发育基因。
Key messageA new rice floral organ mutantbh1, had a negative effect on grain yield.BH1was fine mapped to 87.5kb on chr2. A 55kb chromosome segment was deleted inbh1.AbstractThe cereal spikelet is enclosed by the lemma and palea. The lemma and palea of the floral mutant designatedbh1, a selection from a T-DNA library generated from the rice cultivar Asominori, takes on an abnormal curve-shaped appearance only late in floral development, finally forming a beak-shaped hull. The mutation had a negative effect on thousand grain weight, seed set rate and germination rate. Genetic analysis indicated that the mutant phenotype was determined by a single recessive gene. Through map-based approach,BH1gene was finally located to a ~87.5-kbp region on the long arm of chromosome 2. An analysis of the gene content of this region indicated that the mutation involves the loss of a 55-kbp stretch, harboring four open reading frames. Transcription profiling based on qRT-PCR revealed that the genesOsMADS1,OsMADS14,OsMADS15,OsMADS18,REP1,CFO1, andDL, all of which are also involved in lemma and palea development and identity specification, were down-regulated in thebh1mutant.BH1is therefore an important floral organ development gene.