Inhibitory effect of grapefruit seed extract (GSE) on avian pathogens
Inhibitory effect of grapefruit seed extract (GSE) on avian pathogens
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DOI：
10.1292/jvms.18-0754
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发表时间：
2019-03-01
影响因子：
1.2
通讯作者：
Takehara, Kazuaki
中科院分区：
文献类型：
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作者：
Komura, Miyuki;Suzuki, Mayuko;Takehara, Kazuaki
研究了西柚籽提取物(GSE)对禽流感病毒(AIV)、新城疫病毒(NDV)、传染性法氏囊病病毒(IBDV)、婴儿沙门氏菌(SI)和大肠杆菌(EC)的抑制作用。原GSE含0.24%苯扎氯铵，0.0025%苯扎氯铵溶液不能灭活细菌。本研究评价了稀释的GSE(X100、X500和X1000加重蒸馏水)对选定的病毒和细菌的抑制活性。将GSE溶液与病原体孵育一段时间后，对剩余的病毒进行滴定，并计算细菌菌落。在有机物质-5%胎牛血清(FBS)存在下，分别在1 cm和30 cm的距离喷洒试验溶液，以喷雾的形式测试GSE的效果。此外，在无纺布上测试了GSE对衣服上细菌的杀灭效果。GSEx100在5%胎牛血清条件下也能降低禽流感病毒和新城疫病毒的滴度。IBDV对GSE表现出高度抗性。GSEx1000在5秒内使SI和EC失活，即使在5%FBS存在的情况下也是如此。该消毒剂在距离30厘米处仍能保持其喷雾形式的效果。GSE对接种在织物上的SI和EC也有一定的抑制作用。GSE是一种潜在的新型病毒和细菌消毒剂，即使在很短的接触时间内也有效。
The inhibitory activities of grapefruit seed extract (GSE) on avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), Salmonella Infantis (SI) and Escherichia coli (EC) were evaluated. Original GSE contained 0.24% benzalkonium chloride (BZC), however, 0.0025% BZC solution could not inactivate bacteria. The activity of diluted GSE (x100, x500 and x1,000 with redistilled water) against selected viruses and bacteria was evaluated in this study. The GSE solutions were incubated with the pathogens over a period of time after which the remaining viruses were titrated and the bacterial colonies were counted. In the presence of organic material-5% fetal bovine serum (FBS), the test solutions were sprayed at 1 cm and 30 cm distances to test the efficacy of GSE in a spray form. Furthermore, the efficacy of GSE against bacteria on clothes was tested using non-woven cloth. GSEx100 reduced the viral titer of both AIV and NDV even in 5% FBS condition. IBDV showed high resistance to GSE. GSEx1,000 inactivated both SI and EC within 5 sec, even in the presence of 5% FBS. The disinfectant was able to maintain its efficacy in the spray form at 30 cm distance. GSE was also effective against SI and EC inoculated on fabric. GSE is a potential novel disinfectant against viruses and bacteria, effective even within a short contact time.