Cortical electrical stimulation alone enhances functional recovery and dendritic structures after focal cerebral ischemia in rats
Cortical electrical stimulation alone enhances functional recovery and dendritic structures after focal cerebral ischemia in rats
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DOI：
10.1016/j.brainres.2009.11.022
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发表时间：
2010-01
期刊：
影响因子：
2.9
通讯作者：
Qin Zhou;Qian Zhang;Xueqing Zhao;Yanwen Y Duan;Y. Lu;Chengyan Li;Tao Li
中科院分区：
文献类型：
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作者：
Qin Zhou;Qian Zhang;Xueqing Zhao;Yanwen Y Duan;Y. Lu;Chengyan Li;Tao Li
采用全植入式低频脉冲串皮层电刺激（CES）装置，观察了CES对大脑中动脉闭塞（MCAO）大鼠行为恢复和树突状结构表面密度的影响。在大鼠MCAO后，磁共振成像（MRI）用于确认皮层梗死并确定在梗死周围皮层上植入刺激电极的位置。在MCAO后第6天植入器械，持续CES，持续16天。刺激程序包括上午（0.65 mA，0.13 μC/相）和下午（0.5 mA，0.1 μC/相）两个持续半小时的会话。刺激器在重复的10-s块中以50 Hz、20 Hz和5 Hz的各种频率递送双相电荷平衡脉冲（脉宽=200 μs）。CES组大鼠（n=12）恢复术前体重（BW）水平的时间比无刺激（NS）组（n=9）短得多。在行为测试中，与NS组相比，CES组的大鼠表现出更大的功能恢复。此外，功能改善与梗死周围皮质中对微管相关蛋白2（MAP 2）免疫反应的树枝状突起表面密度的增加相一致。这些结果表明，完全植入CES设备的可行性和新的刺激方案单独改善大鼠局灶性脑缺血后的功能结果和皮质神经元结构可塑性的有效性。
Using a fully implanted cortical electrical stimulation (CES) device with low-frequency burst impulse train, we investigated the effects of CES alone on behavioral recovery and surface density of dendritic structure in a rat model of middle cerebral artery occlusion (MCAO). After MCAO in rats, magnetic resonance imaging (MRI) was used to confirm cortex infarction and to identify a location for implantation of stimulating electrode over the peri-infarct cortex. The device was implanted on the 6th day after MCAO with CES then lasting for 16 days. The stimulation program consisted of two sessions lasting half an hour in the morning (0.65 mA, 0.13 μC/phase) and in the afternoon (0.5 mA, 0.1 μC/phase). The stimulator delivered biphasic charge balanced pulses (pulse width=200 μs) with various frequencies of 50 Hz, 20 Hz and 5 Hz in repeated 10-s blocks. Rats in the CES group (n=12) spend a much shorter time to regain preoperative levels of body weight (BW) than those in the no stimulation (NS) group (n=9). In behavioral tests, the rats in the CES group showed greater functional recovery compared to the NS group. Moreover, the functional improvement coincided with an increase in surface density of dendritic processes immunoreactive to microtubule-associated protein 2 (MAP2) in peri-infarct cortex. These results suggest the feasibility of the fully implanted CES device and the efficacy of the new stimulation protocol alone to improve functional outcome and cortical neuronal structural plasticity following focal cerebral ischemia in rats.