The protein mutant database
The protein mutant database
复制标题
DOI：
10.1093/nar/27.1.355
复制
发表时间：
1999-01-01
影响因子：
14.9
通讯作者：
Nishikawa, K
中科院分区：
文献类型：
--
作者：
Kawabata, T;Ota, M;Nishikawa, K
目前，蛋白质突变体数据库（PMD）包含超过81 000个突变体，包括从大约10 000篇文章中提取的各种蛋白质的人工和天然突变体。我们最近开发了一个功能强大的查看和检索系统（http：pmd.ddbj.nig.ac.jp），该系统与序列和三级结构数据库集成。该系统具有以下特征：（i）根据条目中描述的信息与整合的野生型蛋白质的序列数据一起自动生成后显示突变序列。这是一个方便的特征，因为它允许人们看到改变的氨基酸的位置（ii）对于已经通过实验确定了3D结构的那些蛋白质，以不同的颜色显示3D结构以显示突变位点;（iii）可以用任何查询序列进行针对PMD的序列同源性搜索;（iv）可以显示同源序列的突变的汇总，其显示在整个PMD中记录的蛋白质的某个位点处的所有突变。
Currently the protein mutant database (PMD) contains over 81 000 mutants, including artificial as well as natural mutants of various proteins extracted from about 10 000 articles. We recently developed a powerful viewing and retrieving system (http://pmd.ddbj.nig.ac.jp), which is integrated with the sequence and tertiary structure databases. The system has the following features: (i) mutated sequences are displayed after being automatically generated from the information described in the entry together with the sequence data of wild-type proteins integrated. This is a convenient feature because it allows one to see the position of altered amino acids (shown in a different color) in the entire sequence of a wild-type protein; (ii) for those proteins whose 3D structures have been experimentally determined, a 3D structure is displayed to show mutation sites in a different color; (iii) a sequence homology search against PMD can be carried out with any query sequence; (iv) a summary of mutations of homologous sequences can be displayed, which shows all the mutations at a certain site of a protein, recorded throughout the PMD.