Characterisation of Mal d 1-related genes in Malus
Characterisation of Mal d 1-related genes in Malus
复制标题
DOI：
10.1007/s11103-004-0904-9
复制
发表时间：
2004-05-01
影响因子：
5.1
通讯作者：
MacRae, EA
中科院分区：
文献类型：
--
作者：
Beuning, LL;Bowen, JH;MacRae, EA
研究表明，在苹果的一个基因型中，至少存在15个与Mal d 1相关的基因（PR 10）。我们对栽培品种“皇家嘎拉”的cDNA文库进行了测序，并鉴定了12个Mal d I家族成员，包括先前报道的Mal d l B和Mal d l d，先前报道的Mal d la的等位基因变体。8个Mal d I基因产物在树成熟果实中表达，在皮层或皮肤中，并且其中大多数也在叶片中表达，以响应苹果黑星病菌（Venturia inaequalis）的挑战。从大量不同组织中鉴定出Mal d I基因产物。通过标准参数测量的成熟度显示不能预测能够与特异性单克隆抗体5118结合的蛋白质的量（先前显示与Mal d Ib和a/d中的变应原表位结合）或存在的Mal d I mRNA的量。Mal d Id和Mal d lb在“皇家加拉”中表达量最高，尤其是在果皮中，这些异构体在其它品种和种中也占优势。然而，基因型，不同的相对优势的Mal d lb和Mal d Id。主要表达的Mal d I基因在成熟的苹果果实在体内翻译成蛋白质和蛋白质结合的抗体被发现在所有品种和物种检查。鉴定了与5 H8抗体结合的新的Mal d I蛋白。至少有两个新的亚家族已被确定，虽然一些结构差异的预测组之间的异构体，P-环基序是相同的，除了两个异构体。建议在植物细胞内信号传导的作用，并在体外表达的异构体应有助于评估其在疾病，过敏反应，衰老和核苷酸，细胞分裂素和油菜素类固醇结合的相对作用。
It has been suggested that there are at least 15 Mal d 1-related (PR10) genes in one genotype of apple (Malus x domestica Borkh.). We sequenced cDNA libraries of cultivar 'Royal Gala' and identified 12 members of the Mal d I family, including the previously reported Mal d l b and Mal d l d, an allelic variant of the previously reported Mal d la. Eight Mal d I gene products were expressed in tree-ripened fruit, in either the cortex or the skin, and most of these were also expressed in leaves in response to challenge with Venturia inaequalis-a fungal disease of apple. Mal d I gene products were identified from a large number of different tissues. Degree of ripeness as measured by standard parameters was shown not to predict either the amount of protein able to bind to a specific monoclonal antibody 5118, previously shown to bind to an allergenic epitope in Mal d lb and a/d, or the amount of Mal d I mRNA present. Mal d Id and Mal d lb were the most highly expressed isoforms in 'Royal Gala', particularly in the skin of fruit, and these isoforms were also predominant in other cultivars and species of apple. Genotypes, however, differed in relative predominance of Mal d lb and Mal d Id. The predominantly expressed Mal d I genes in ripe apple fruit were translated in vivo into proteins and proteins binding to the antibody were found in all cultivars and species examined. New Mal d I proteins were identified that bound to the 5H8 antibody. At least two new subfamilies have been identified, and while some structural differences are predicted between groups of isoforms, the P-loop motif is identical in all except two isoforms. A role in intracellular signalling in plants is suggested and in vitro expression of the isoforms should help in assessing their relative roles in disease, allergic responses, senescence and nucleotide-, cytokinin- and brassinosteroid-binding.