Thermostability of biological systems: fundamentals, challenges, and quantification.
Thermostability of biological systems: fundamentals, challenges, and quantification.
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DOI：
10.2174/1874120701105010047
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
He X
中科院分区：
文献类型：
--
作者：
He X
本文综述了生物系统在较宽温度范围内(从低温到高温)在工程设计/理解热稳定性方面的基本原理和挑战。首先介绍了生物热稳定性工程在现代医学中用于销毁或稳定有用的生物制剂以治疗疾病的应用。从分子水平、细胞水平和组织/器官水平对低温和高温的生物响应进行了综述，以了解热(低温和高温)损伤的机制及其量化。然后总结了量化不同应用的热物理过程的方法，考虑了血液灌流、新陈代谢、水在细胞膜上的传输以及水的相变(平衡和非平衡，如结冰和玻璃化转变)的影响。本文最后对生物系统热稳定性工程的现状和未来前景进行了总结。
This review examines the fundamentals and challenges in engineering/understanding the thermostability of biological systems over a wide temperature range (from the cryogenic to hyperthermic regimen). Applications of the bio-thermostability engineering to either destroy unwanted or stabilize useful biologicals for the treatment of diseases in modern medicine are first introduced. Studies on the biological responses to cryogenic and hyperthermic temperatures for the various applications are reviewed to understand the mechanism of thermal (both cryo and hyperthermic) injury and its quantification at the molecular, cellular and tissue/organ levels. Methods for quantifying the thermophysical processes of the various applications are then summarized accounting for the effect of blood perfusion, metabolism, water transport across cell plasma membrane, and phase transition (both equilibrium and non-equilibrium such as ice formation and glass transition) of water. The review concludes with a summary of the status quo and future perspectives in engineering the thermostability of biological systems.