BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis.
BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis.
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BMSC衍生的外泌体miR-29 a促进血管生成和骨生成。
DOI：
10.3389/fcell.2020.608521
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发表时间：
2020
影响因子：
5.5
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Lu GD;Cheng P;Liu T;Wang Z
在骨建模和重建过程中，血管生成和骨生成紧密耦合。本文报道了骨髓间充质干细胞（BMSC）来源的外泌体miR-29 a在体内外促进血管生成和成骨的作用。BMSC来源的外泌体（BMSCs-Exos）可被人脐静脉内皮细胞（HUVECs）摄取并促进HUVECs的增殖、迁移和管腔形成。miRNA-29 a在BMSCs-Exos中表达水平较高，并可被转运至HUVECs中调节血管生成。VASH 1被确定为miR-29 a的直接靶点，介导BMSC来源的外泌体miR-29 a对血管生成的影响。更有趣的是，来自工程化BMSC的miR-29 a负载的外泌体（miR-29 a-loaded BMSCs-Exos）显示出体内促进血管生成和骨生成的强大能力。综上所述，这些发现表明BMSC衍生的外泌体miR-29 a调节血管生成和骨生成，并且miR-29 a负载的BMSCs-Exos可能作为骨质疏松症的潜在治疗靶点。
Angiogenesis and osteogenesis are tightly coupled during bone modeling and remodeling processes. Here we reported that bone marrow mesenchymal stem cell (BMSC)-derived exosomal miR-29a promotes angiogenesis and osteogenesis in vitro and in vivo. BMSC-derived exosomes (BMSCs-Exos) can be taken up by human umbilical vein endothelial cells (HUVECs) and promote the proliferation, migration, and tube formation of HUVECs. MiRNA-29a level was high in BMSCs-Exos and can be transported into HUVECs to regulate angiogenesis. VASH1 was identified as a direct target of miR-29a, mediating the effects of BMSC-derived exosomal miR-29a on angiogenesis. More interestingly, miR29a-loaded exosomes from engineered BMSCs (miR-29a-loaded BMSCs-Exos) showed a robust ability of promoting angiogenesis and osteogenesis in vivo. Taken together, these findings suggest that BMSC-derived exosomal miR-29a regulates angiogenesis and osteogenesis, and miR-29a-loaded BMSCs-Exos may serve as a potential therapeutic target for osteoporosis.