PHASE-SHIFTING OF THE CIRCADIAN CLOCK BY INDUCTION OF THE DROSOPHILA PERIOD PROTEIN
PHASE-SHIFTING OF THE CIRCADIAN CLOCK BY INDUCTION OF THE DROSOPHILA PERIOD PROTEIN
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DOI：
10.1126/science.8284676
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发表时间：
1994-01-14
期刊：
影响因子：
56.9
通讯作者：
ROSBASH, M
中科院分区：
文献类型：
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作者：
EDERY, I;RUTILA, JE;ROSBASH, M
事实上，所有生物体都表现出昼夜（24小时）节律，由定义不清的内源性起搏器或时钟控制。几条线索的证据表明，果蝇时期基因产物PER是一个时钟组件。如果PER是计时机制的核心，其浓度的短暂增加将导致时钟相位的稳定偏移。因此，携带PER的热诱导拷贝的转基因果蝇经受温度脉冲。这种治疗引起了运动活动昼夜节律的长期相移，这一结果支持PER是真正的时钟成分的论点。
Virtually all organisms manifest circadian (24-hour) rhythms, governed by an ill-defined endogenous pacemaker or clock. Several lines of evidence suggest that the Drosophila melanogaster period gene product PER is a clock component. If PER were central to the time-keeping mechanism, a transient increase in its concentration would cause a stable shift in the phase of the clock. Therefore, transgenic flies bearing a heat-inducible copy of PER were subjected to temperature pulses. This treatment caused long-lasting phase shifts in the locomotor activity circadian rhythm, a result that supports the contention that PER is a bona fide clock component.