MACROMOLECULE SYNTHESIS IN TEMPERATURE-SENSITIVE MUTANTS OF YEAST
MACROMOLECULE SYNTHESIS IN TEMPERATURE-SENSITIVE MUTANTS OF YEAST
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DOI：
10.1128/jb.93.5.1662-1670.1967
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发表时间：
1967-01-01
影响因子：
3.2
通讯作者：
HARTWELL, LH
中科院分区：
文献类型：
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作者：
HARTWELL, LH
从酿酒酵母中分离出约400个温度敏感突变体。突变体不能形成殖民地在丰富媒体36 C,但正常增长,或几乎如此,在23 C突变体检测失去生存能力,形态的变化,增加细胞数量,并能合成蛋白质,核糖核酸(RNA)和脱氧核糖核酸(DNA)后,从23日转移到36 C突变被发现导致优惠亏损的能力进行蛋白质合成,合成RNA, DNA合成,细胞分裂,或者细胞壁形成。构建温度敏感突变的杂合二倍体细胞，并测试其在36℃下形成菌落的能力，鉴定出4个对野生型等位基因有利的突变。
Approximately 400 temperature-sensitive mutants of Saccharomyces cerevisiae were isolated. The mutants were unable to form colonies on enriched media at 36 C, but grew normally, or nearly so, at 23 C. The mutants were tested for loss of viability, change in morphology, increase in cell number, and the ability to synthesize protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA) after a shift from 23 to 36 C. Mutations were found which resulted in a preferential loss of ability of carry out protein synthesis, RNA synthesis, DNA synthesis, cell division, or cell wall formation. Diploid cells heterozygous for the temperature-sensitive mutations were constructed and tested for their ability to form colonies at 36 C. Four mutations dominant to their wild-type allele were identified.