Post-acquisition hippocampal NMDA receptor blockade sustains retention of spatial reference memory in Morris water maze
Post-acquisition hippocampal NMDA receptor blockade sustains retention of spatial reference memory in Morris water maze
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DOI：
10.1016/j.bbr.2013.11.016
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发表时间：
2014-02
影响因子：
2.7
通讯作者：
Keisuke Shinohara;T. Hata
中科院分区：
文献类型：
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作者：
Keisuke Shinohara;T. Hata
一些研究表明，海马区N-甲基-d-天冬氨酸型谷氨酸受体(NMDAR)是空间参考记忆获得所必需的，但不是保持所必需的。相反，一些研究表明，获取后重复注射NMDAR拮抗剂有助于在放射状迷宫任务中保持空间参考记忆。在本研究中，我们研究了海马NMDAR在Morris水迷宫空间参考记忆保持中的作用。实验一，训练后24小时(4次/d，共4天)，将d-AP5慢性注入大鼠海马区，连续5天。在随后的探测测试中(训练后7天)，我们发现，与Chance水平相比，注入d-AP5的大鼠在目标象限停留的时间明显更长，而对照组的大鼠没有这样做。在实验2中，d-AP5在训练后1天(即刻)或7天(延迟)注入海马区。在探针测试中，在保留间隔13天后，立即输液以类似于实验1的方式促进了表现，而延迟输液则没有。这些发现表明，海马区NMDAR在空间参考记忆的退化中起着重要作用。
Several studies have demonstrated that the hippocampalN-methyl-d-aspartate type glutamate receptors (NMDARs) are necessary for the acquisition but not the retention of spatial reference memory. In contrast, a few studies have shown that post-acquisition repetitive intraperitoneal injections of an NMDAR antagonist facilitate the retention of spatial reference memory in a radial maze task. In the present study, we investigated the role of hippocampal NMDARs in the retention of spatial reference memories in Morris water maze. In Experiment 1, 24 h after training (4 trials/day for 4 days),d-AP5 was chronically infused into the hippocampus of rats for 5 days. In the subsequent probe test (seven days after training), we found that rats infused withd-AP5 spent a significantly longer time in the target quadrant compared to chance level, whereas rats in the control group did not. In Experiment 2,d-AP5 was infused into the hippocampus 1 (immediate) or 7 (delayed) days after the training session. In the probe test, following the retention interval of 13 days, immediate infusion facilitated the performance in a manner similar to Experiment 1, whereas the delayed infusion did not. These findings suggest that hippocampal NMDARs play an important role in the deterioration of spatial reference memory.