Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase
Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase
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DOI：
10.3390/s19071677
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发表时间：
2019-04-01
期刊：
影响因子：
3.9
通讯作者：
Krause, Steffi
中科院分区：
文献类型：
--
作者：
Ahmad, Norlaily;Colak, Burcu;Krause, Steffi
肽交联聚乙二醇水凝胶已广泛应用于药物缓释和组织工程。然而，使用这种材料作为检测胶原酶的生物传感器还没有被探索。蛋白酶在类风湿性关节炎和骨关节炎等疾病的病理学中起关键作用。使用可降解水凝胶膜形式检测这类酶作为用于疾病监测的即时装置是有希望的。在这项研究中，蛋白酶生物传感器的基础上开发的肽交联聚（乙二醇）水凝胶膜的降解和证明用于检测胶原酶。将水凝胶沉积在涂金的石英晶体上，并使用石英晶体微天平（QCM）监测它们在胶原酶存在下的降解。该生物传感器被证明在不到10分钟内响应于2和2000 nM之间的浓度，检测下限为2 nM。
Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM.