Presence of B cell progenitors in the thymus.
Presence of B cell progenitors in the thymus.
复制标题
DOI：
10.4049/jimmunol.158.9.4193
复制
发表时间：
1997-05
影响因子：
4.4
通讯作者：
S. Mori;M. Inaba;A. Sugihara;S. Taketani;H. Doi;Y. Fukuba;Y. Yamamoto;Y. Adachi;K. Inaba;S. Fukuhara;S. Ikehara
中科院分区：
文献类型：
--
作者：
S. Mori;M. Inaba;A. Sugihara;S. Taketani;H. Doi;Y. Fukuba;Y. Yamamoto;Y. Adachi;K. Inaba;S. Fukuhara;S. Ikehara
我们描述了胸腺中的B细胞祖细胞。虽然成熟的胸腺B细胞是表面IG+，B220（高），但胸腺中的CD 43-，B细胞祖细胞被发现是sIg-，B220（中）和CD 43+。当通过PCR扩增来自分选的B细胞祖细胞的DNA时，大多数祖细胞显示重排的IG D-J和V-D-J模式，印迹并探测。当从胸腺中纯化B细胞祖细胞并将其胸腺内注射到IG重链（Igh）同种异型不同受体中时，在胸腺中检测到携带供体型Igh 6的B细胞，但在外周中未检测到。由这些祖细胞产生的胸腺B细胞是CD 5+细胞，尽管存在广泛的CD 5分子表达。此外，从接种了B细胞祖细胞的胸腺纯化的B细胞能够在CD 40配体加IL-10的影响下分化为Ab形成细胞，并分泌具有供体Igh 6同种异型的IgM。因此，我们澄清了B细胞祖细胞在胸腺中的存在，也表征了表面标记物及其发育功能。
We characterize B cell progenitors in the thymus. Although mature thymic B cells are surface Ig+, B220(high), but CD43-, B cell progenitors in the thymus were found to be slg-, B220(med), and CD43+. Most progenitors showed rearranged Ig D-J and V-D-J patterns when DNA from sorted B cell progenitors was amplified by PCR, blotted, and probed. When B cell progenitors were purified from the thymus and injected intrathymically into Ig heavy chain (Igh) allotype-disparate recipients, B cells bearing donor-type Igh6 were detected in the thymus but not in the periphery. Thymic B cells generated from these progenitors were CD5+ cells, although there was a broad range of expression of CD5 molecules. Furthermore, B cells purified from thymus that had been inoculated with B cell progenitors were able to differentiate into Ab-forming cells under the influence of CD40 ligand plus IL-10, and secreted IgM with donor Igh6 allotype. We thus clarified the existence of B cell progenitors in the thymus and also characterized the surface markers and their developmental functions.