Study on the controlling effects of pokeweed extracts against tobacco mosaic virus
Study on the controlling effects of pokeweed extracts against tobacco mosaic virus
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发表时间：
2005
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通讯作者：
Ma Xiao;Shuijin Zhu;D. Wei;Yongqiang Zhang
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作者：
Ma Xiao;Shuijin Zhu;D. Wei;Yongqiang Zhang
植物病毒病是仅次于真菌的重要植物病害，每年给农业生产造成巨大损失，采用3种美洲商陆提取物和2种对照农药，进行了几种处理的抗TMV活性研究，结果表明，2%宁南霉素、60%毒克星含片和美洲商陆甲醇提取物对TMV均有较好的抑制效果。在200和100 μg·mL~（-1）浓度下，上述3种处理的抑菌效果均在80%以上，其中美洲商陆甲醇提取物的抑菌效果与60%毒克星含片相当。当浓度为200 μg·mL~（-1）时，美洲商陆甲醇提取物与TMV混合后立即接种，抑制率为88.56%，与60%毒克星含片和2%宁南霉素相当。对烟草TMV的几种处理试验表明，接种前用2%宁南霉素、60%毒克星含片或美洲商陆甲醇提取物处理烟草，可抑制烟草TMV初侵染，相对防效分别为94.39%、92.52%和87.85%，到目前为止，植株个体病害的发生时间推迟了3- 5d。
Plant virus was an important plant diseases only inferior to fungi and caused great loss to agriculture products annually.Using three pokeweed extracts and two CK pesticides,the research of anti-TMV activity of several treatments was carried out.The results showed that 2 % ningnanmycin, 60 % dukexing effervesce troche and pokeweed methanol extracts all exhibited better inhibition effects against TMV. At 200 and 100 μg·mL~(-1),all three treatments above mentioned showed all above 80 % inhibition effects, especially,the pokeweed methanol extract inhibition effects was the same as that of 60 % dukexing effervesce troche. At 200 μg·mL~(-1),pokeweed methanol extracts mixed with TMV inoculation promptly after mixing, the inhibition rate was 88.56 % and was almost up to 60 % dukexing effervesce troche and 2 % ningnanmycin. The experiments of several treatments against TMV infectivity showed that Nicotiana glutinosa treated by using 2 % ningnanmycin, 60 % dukexing effervesce troche or pokeweed methanol extract before inoculation could inhibite primary infection.TMV infectivity could be controlled and the relative efficacy were 94.39 %, 92.52 % and 87.85 %, and so far the occurrence time of plant individual disease was delayed for 3-5 d.