Identification of Epstein-Barr Virus Replication Proteins in Burkitt's Lymphoma Cells.
Identification of Epstein-Barr Virus Replication Proteins in Burkitt's Lymphoma Cells.
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DOI：
10.3390/pathogens4040739
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发表时间：
2015-10-29
期刊：
影响因子：
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通讯作者：
Sinclair AJ
中科院分区：
文献类型：
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作者：
Traylen C;Ramasubramanyan S;Zuo J;Rowe M;Almohammad R;Heesom K;Sweet SM;Matthews DA;Sinclair AJ
描述用于复制癌症相关病毒Epstein-Barr病毒(EBV)的机制的工作模型部分源于与疱疹病毒家族其他成员的比较。EBV基因组中的许多基因在疱疹病毒家族中是同源的。已发表的EBV基因组在其裂解复制周期中的转录组数据显示了广泛的转录，但对蛋白质的鉴定是有限的。我们采用了一种全球蛋白质组学方法来识别在EBV裂解复制周期中表达的病毒蛋白。我们结合了一种富集法来分离进行EBV裂解复制的细胞，并将SILAC标记与质谱学相结合，鉴定了在EBV裂解复制周期中表达的病毒和宿主蛋白。最常见的病毒蛋白是DNA复制机制的两个组成部分，即单链DNA结合蛋白BALF2、DNA聚合酶辅助蛋白BMRF1和病毒核糖核苷二磷酸还原酶的两个亚基(BORF2和BaRF1)。另外还检测到42个EBV裂解循环蛋白。这为许多EBV裂解复制周期蛋白提供了蛋白质组学鉴定，也鉴定了翻译后修饰。
The working model to describe the mechanisms used to replicate the cancer-associated virus Epstein-Barr virus (EBV) is partly derived from comparisons with other members of the Herpes virus family. Many genes within the EBV genome are homologous across the herpes virus family. Published transcriptome data for the EBV genome during its lytic replication cycle show extensive transcription, but the identification of the proteins is limited. We have taken a global proteomics approach to identify viral proteins that are expressed during the EBV lytic replication cycle. We combined an enrichment method to isolate cells undergoing EBV lytic replication with SILAC-labeling coupled to mass-spectrometry and identified viral and host proteins expressed during the EBV lytic replication cycle. Amongst the most frequently identified viral proteins are two components of the DNA replication machinery, the single strand DNA binding protein BALF2, DNA polymerase accessory protein BMRF1 and both subunits of the viral ribonucleoside-diphosphate reductase enzyme (BORF2 and BaRF1). An additional 42 EBV lytic cycle proteins were also detected. This provides proteomic identification for many EBV lytic replication cycle proteins and also identifies post-translational modifications.