Gene Expression Profiling and Bioinformatic Analysis of Rabbit Basilar Artery after Experimental Subarachnoid Hemorrhage
Gene Expression Profiling and Bioinformatic Analysis of Rabbit Basilar Artery after Experimental Subarachnoid Hemorrhage
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DOI：
10.4172/2155-9562.1000201
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发表时间：
2014-03
期刊：
影响因子：
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通讯作者：
Y. Kikkawa;S. Matsuo;M. Hokama;Ryota Kurogi;A. Nakamizo;M. Mizoguchi;Tomio Sasaki
中科院分区：
文献类型：
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作者：
Y. Kikkawa;S. Matsuo;M. Hokama;Ryota Kurogi;A. Nakamizo;M. Mizoguchi;Tomio Sasaki
目的：脑动脉蛛网膜下腔出血（SAH）后血管事件（包括脑血管痉挛）发生的分子机制尚不清楚。本研究采用兔SAH模型，观察脑动脉基因表达的时程变化，并对差异表达基因进行生物信息学分析。方法：兔脑出血后第3、5、7天取基底动脉。利用Agilent兔寡核苷酸芯片检测兔基底动脉基因表达的变化，并通过不可逆途径分析（IPA）对数据进行分析。结果：在研究的43,623个基因中，有1,121个基因在至少1个时间点上有差异表达。我们发现，倍数变化的数量、幅度和基因表达模式在第3天动态变化最大，而基底动脉狭窄在第5天变得最严重。在微阵列数据集分析IPA显示，25个生物学功能的差异表达基因被显着上调。结论：我们的研究结果是基于基因表达分析，然后进行生物信息学分析，这可能为各种假定的血管事件（包括SAH后脑血管痉挛）发病机制之间的相互联系提供了一个简单的基础。
Objective: The molecular mechanisms which contribute to the development of vascular events including cerebral vasospasm after subarachnoid hemorrhage (SAH) in cerebral artery remain to be elucidated. In this study, we investigated the time course of changes in the gene expression of cerebral artery using rabbit SAH model and performed bioinformatic analysis of differentially expressed genes. Methods: Rabbit basilar arteries were harvested on days 3, 5, and 7 after initial hemorrhage. Changes in gene expression of the rabbit basilar artery were investigated by using Agilent rabbit oligo microarrays and analyzed the data by Ingenuity Pathway Analysis (IPA). Results: Among investigated 43,623 genes, 1,121 genes were differentially expressed at least 1 time point. We found that the number, magnitude of fold change, and gene expression pattern were most dynamically changed on day 3, whereas narrowing of the basilar artery became most severe on day 5. In microarray datasets analyzed by IPA revealed that 25 biological functions identified from differentially expressed genes were significantly upregulated. Conclusion: Our findings that were based on gene expression analysis followed by bioinformatic analysis may provide a simple basis to interlink the various presumed pathogenesis of vascular events including cerebral vasospasm after SAH.