Independent expansion of the keratin gene family in teleostean fish and mammals: An insight from phylogenetic analysis and radiation hybrid mapping of keratin genes in zebrafish
Independent expansion of the keratin gene family in teleostean fish and mammals: An insight from phylogenetic analysis and radiation hybrid mapping of keratin genes in zebrafish
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DOI：
10.1016/j.gene.2005.09.016
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发表时间：
2006-03-01
期刊：
影响因子：
3.5
通讯作者：
Ekker, M
中科院分区：
文献类型：
--
作者：
Padhi, BK;Akimenko, MA;Ekker, M
将斑马鱼角蛋白基因的序列和染色体分布与其他鱼类和哺乳动物的角蛋白基因进行比较，以深入了解该基因家族在脊椎动物中的进化。通过比较序列分析和辐射杂交作图，我们在斑马鱼基因组中鉴定出了 16 个 I 型角蛋白基因和 7 个 11 型角蛋白基因。这与哺乳动物形成鲜明对比，哺乳动物的 I 型和 11 型角蛋白基因数量相似。角蛋白基因分散在鱼类基因组中，与哺乳动物基因组中的两个角蛋白基因簇形成鲜明对比。与两种河豚的基因相比，斑马鱼 I 型角蛋白基因通过独立串联复制进行了扩展。基于 EST 计数的表达谱表明，斑马鱼的一些串联重复的 I 型角蛋白基因要么经历了亚功能化，要么获得了新的表达域。角蛋白8、角蛋白18和第二种II型角蛋白的染色体排列作为三个基因的簇，在脊椎动物进化中一直保持保守，除了在一些硬骨鱼中三基因簇的重复之外。这与角蛋白基因家族的其他成员形成鲜明对比，这些成员在鱼类和哺乳动物之间独立分化。 (c) 2005 Elsevier B.V. 保留所有权利。
The sequence and chromosomal distribution of keratin genes of zebrafish were compared with that of other fishes and mammals to provide an insight into the evolution of this gene family in vertebrates. By comparative sequence analysis and radiation hybrid mapping, we identified 16 type I and 7 type 11 keratin genes in the zebrafish genome. This contrasts with mammals, where type I and type 11 keratin genes are similar in number. The keratin genes are scattered in the fish genome, contrasting with the two clusters of keratin genes in mammalian genomes. Compared to genes from two species of pufferfish, the zebrafish type I keratin genes underwent an expansion by independent tandem duplications. Expression profiles based on EST counts suggest that some of the tandemly duplicated type I keratin genes from zebrafish either underwent sub-functionalization or acquired new expression domains. The chromosomal arrangement of keratins 8, keratin 18, and a second type I I keratin, as a cluster of three genes, has remained conserved in vertebrate evolution, except for duplication of the three-gene cluster in some teleosts. This contrasts with other members of the keratin gene family, which diverged independently between fish and mammals. (c) 2005 Elsevier B.V. All rights reserved.