The association of the Clock 3111 T/C SNP with lipids and lipoproteins including small dense low-density lipoprotein: results from the Mima study.
The association of the Clock 3111 T/C SNP with lipids and lipoproteins including small dense low-density lipoprotein: results from the Mima study.
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DOI：
10.1186/1471-2350-11-150
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发表时间：
2010-10-21
影响因子：
--
通讯作者：
Sakane N
中科院分区：
文献类型：
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作者：
Tsuzaki K;Kotani K;Sano Y;Fujiwara S;Takahashi K;Sakane N
时钟分子在昼夜节律以及调节外周器官的脂质和葡萄糖代谢中发挥着重要作用。昼夜节律紊乱会导致心脏代谢紊乱。循环中存在小而密的低密度脂蛋白（sdLDL），这是一种脂质代谢异常，部分与生活方式有关，也是心脏代谢疾病的风险参数之一。据报道，时钟基因的 3111 T/C 单核苷酸多态性 (SNP) 与生活方式（包括早/晚偏好）相关。我们研究了 Clock 3111 T/C SNP 是否可能影响脂质和脂蛋白，包括 sdLDL。在 365 名社区居民受试者（170 名男性和 195 名女性，平均年龄 63 ± 14 岁）中，使用荧光等位基因特异性 DNA 引物测定系统对 3111 T/C SNP 进行了基因分型。使用 Lipoprint 系统通过电泳分离脂蛋白来测量 sdLDL 水平。 Clock 3111 C 等位基因的频率为 0.14。肥胖受试者和非肥胖受试者的 sdLDL 面积没有差异。在 T/T 纯合子携带者中，sdLDL 面积显着高于 C 等位基因携带者（T/C 或 C/C）（1.7 ± 3.4 vs. 0.8 ± 1.9%；p < 0.05）。多元回归分析显示，sdLDL 面积与 Clock 3111 T/C SNP 显着负相关（β = -0.114，p < 0.05），与年龄、性别、体重指数和运动习惯无关。我们的研究结果表明，时钟 3111 T/C SNP 可能与 sdLDL 的存在有关。
The clock molecule plays major roles in circadian rhythmicity and regulating lipid and glucose metabolism in peripheral organs. Disruption of the circadian rhythm can lead to cardiometabolic disorders. The existence of small dense low-density lipoprotein (sdLDL) in the circulation, an abnormality of lipid metabolism, in part associated with lifestyle, is also one of risk parameters for cardiometabolic disorders. The 3111 T/C single nucleotide polymorphism (SNP) of the Clock gene has been reported to be associated with lifestyle including morning/evening preference. We investigated whether the Clock 3111 T/C SNP may affect lipids and lipoproteins including sdLDL. In 365 community-dwelling subjects (170 men and 195 women, mean age 63 ± 14 years), the 3111 T/C SNP was genotyped using a fluorescent allele-specific DNA primer assay system. The levels of sdLDL were measured with the electrophoretic separation of lipoproteins employing the Lipoprint system. The frequency of the Clock 3111 C allele was 0.14. The area of sdLDL did not differ between the subjects with obesity and those without. In carriers of T/T homozygotes, the area of sdLDL was significantly higher compared with carriers of the C allele (T/C or C/C) (1.7 ± 3.4 vs. 0.8 ± 1.9%; p < 0.05). A multiple regression analysis showed that the area of sdLDL was significantly and negatively correlated with the Clock 3111 T/C SNP (β = -0.114, p < 0.05), independently of age, sex, body mass index, and exercise habits. Our findings indicated that the Clock 3111 T/C SNP might be associated with the existence of sdLDL.