Crystallization of Proteins at Ultralow Supersaturations Using Novel Three-Dimensional Nanotemplates
Crystallization of Proteins at Ultralow Supersaturations Using Novel Three-Dimensional Nanotemplates
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DOI：
10.1021/cg201190c
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发表时间：
2012-04-01
影响因子：
3.8
通讯作者：
Heng, Jerry Y. Y.
中科院分区：
文献类型：
--
作者：
Shah, Umang V.;Allenby, Mark C.;Heng, Jerry Y. Y.
一系列新颖的三维(3D)纳米模板已经被开发出来，它们基于感兴趣的蛋白质来调节表面介孔和表面化学，以促进蛋白质的结晶。五个模型蛋白质体系的结晶被报道为迄今报道的最低蛋白质或沉淀剂浓度。只有在制备3D纳米模板的过程中结合使用最佳孔径和适当的表面化学成分，才有可能实现这些改进。这一策略的成功可以归功于有序纳米模板的特定设计，该模板基于蛋白质的已知物理化学性质，与以往基于通用核试剂的方法不同，它为蛋白质结晶提供了一种替代的靶向策略。蛋白质修饰纳米模板的使用将可能为高价值蛋白质的结晶和结构测定开辟新的机会，以及在下游生物加工中分离和纯化蛋白质的机会。
A series of novel three-dimensional (3D) nanotemplates Which have tuned surface mesoporosity and surface chemistry based on the protein of interest have been developed to facilitate protein crystallization. The crystallization of five model proteins systems is reported at hereto the lowest reported protein or precipitant concentrations. These improvements were only possible due to the combined use of optimum pore sizes with appropriate surface chemistries in the preparation of the 3D nanotemplates. The success of this strategy can be ascribed to the specific design of the ordered nanotemplates which are based on known physicochemical properties of the protein and offer an alternate targeted strategy for protein crystallization in contrast to previous methods based on the use of universal nucleants. The use of protein tuned nanotemplates will potentially open up new opportunities for the crystallization and structure determination of high value proteins, as well as opportunities for their separation and purification in downstream bioprocessing.