Changes in antioxidative system and membrane damage of lettuce in response to salinity and boron toxicity
Changes in antioxidative system and membrane damage of lettuce in response to salinity and boron toxicity
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DOI：
10.1016/j.scienta.2007.05.002
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发表时间：
2007-09-11
影响因子：
4.3
通讯作者：
Gunes, A.
中科院分区：
文献类型：
--
作者：
Eraslan, F.;Inal, A.;Gunes, A.
研究了盐和B毒性对生菜生长、主要抗氧化酶（超氧化物歧化酶，SOD，过氧化氢酶，CAT，抗坏血酸过氧化物酶，APX）活性、抗坏血酸、脯氨酸和H2O2、积累和气孔抗性（SR）、丙二醛（MDA）、膜透性（MP）以及钠（Na）、氯（CI）和硼(B)浓度的单独和联合影响。硼毒和盐碱降低了生菜的生长。在B毒性作用下，植株的B浓度升高，但在NaCl的作用下，植株的B浓度显著降低。在B + NaCl和NaCl处理下，钠和氯浓度升高。在NaCl和B + NaCl处理下，膜损伤更为明显。盐度处理显著提高了植株的气孔抗性。脯氨酸和抗坏血酸的积累以B + NaCl处理最高。总的来说，胁迫条件显著提高了H2O2和抗氧化酶（SOD、CAT和APX）活性。结果表明，气孔关闭是莴苣对NaCl和B + NaCl胁迫的重要响应。此外，NaCl和B + NaCl毒性诱导生菜氧化应激，导致脂质过氧化和膜损伤。抗氧化酶活性的增加以及抗坏血酸和脯氨酸的积累参与了克服B-和nacl诱导的氧化应激。(c) 2007 Elsevier B.V.版权所有
Individual and combined effects of salinity and B toxicity on growth, the major antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT; ascorbate peroxidase, APX) activities, ascorbic acid, proline, and H2O2, accumulation, and stomatal resistance (SR), malondialdehyde (MDA), membrane permeability (MP) and the concentrations of sodium (Na), chloride (CI) and boron (B) of lettuce were investigated. Boron toxicity and salinity reduced growth of lettuce plants. Under B toxicity, B concentration of the plants was increased, but in the presence of NaCl, the concentration of B was significantly reduced. Sodium and Cl concentrations were increased in B + NaCl and NaCl treatments. Membrane damage was more pronounced in NaCl and B + NaCl treatments. Stomatal resistance of the plants was significantly increased by salinity treatments. The accumulation of proline and ascorbic acid was the highest in the B + NaCl treatment. In general, stress conditions significantly increased H2O2, and antioxidant enzyme (SOD, CAT and APX) activities. The present results indicate that stomatal closure is an important response of lettuce against NaCl and B + NaCl stress. Furthermore NaCl and B + NaCl toxicity-induced oxidative stress in lettuce resulting in lipid peroxidation and membrane damage. Increased antioxidant enzyme activities and also accumulation of ascorbic acid and proline are involved in order to overcome B- and NaCl-induced oxidative stress. (c) 2007 Elsevier B.V. All rights reserved.