Lovell-Badge R, Takemoto T, Kondoh H. Regulation of mesodermal precursor production by low-level expression of B1 Sox genes in the caudal lateral epiblast
Lovell-Badge R, Takemoto T, Kondoh H. Regulation of mesodermal precursor production by low-level expression of B1 Sox genes in the caudal lateral epiblast
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Lovell-Badge R、Takemoto T、Kondoh H.尾外侧外胚层中 B1 Sox 基因低水平表达对中胚层前体产生的调节
DOI：
10.1016/j.mod.2014.01.003
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Rizzoti K
中科院分区：
文献类型：
--
作者：
Yoshida M;Uchikawa M;Rizzoti K
B1 Sox基因、Sox 2和Sox 3的高表达与早期胚胎中定形神经原基（神经板）的发育有关。然而，在轴向干细胞所在的尾侧外上胚层（CLE）中，Sox 2和Sox 3与Brachyury一起以低水平表达。由于轴向干细胞是神经板和近轴中胚层的双能前体，我们研究了CLE中低水平B1 Sox表达可能调节轴向干细胞命运的可能性。我们结合Sox 3缺失和Sox 2N 1增强子纯合缺失（Sox 2 ΔN1/ΔN1）的遗传条件来降低CLE中B1 Sox的表达。在小鼠胚胎发生的5-7体节阶段，这些遗传操作导致近轴中胚层前体的产量增加约30%，从而导致更大体节的发育。有丝分裂细胞群的分析表明，CLE中B1 Sox表达的减少不会激活细胞增殖，但促进细胞迁移到中胚层隔室。因此，CLE中低水平的B1 Sox表达调节轴向干细胞以将近轴中胚层前体的产生调节到适当的水平。
High expression of the B1Soxgenes,Sox2andSox3,is associated with the development of definitive neural primordia, the neural plates, in early stage embryos. However, in the caudal lateral epiblast (CLE) where axial stem cells reside,Sox2andSox3are expressed at low levels, together withBrachyury. Because axial stem cells are the bipotential precursors of the neural plate and paraxial mesoderm, we investigated the possibility that low-level B1Soxexpression in CLE may regulate the fate of axial stem cells. We combined the genetic conditions ofSox3-null andSox2N1 enhancer homozygous deletion (Sox2ΔN1/ΔN1) to decrease B1Soxexpression in CLE. At 5–7 somite stages of mouse embryogenesis, these genetic manipulations caused approximately 30% higher production of paraxial mesodermal precursors, resulting in the development of larger somites. Analysis of mitotic cell populations suggested that decrease of B1Soxexpression in CLE does not activate cell proliferation but promotes cell migration into the mesodermal compartment. Thus, the low-level B1Soxexpression in CLE regulates axial stem cells to adjust the production of paraxial mesoderm precursors to an appropriate level.