Cadmium-Induced Scale Deformation in Carp (Cyprinus carpio)
Cadmium-Induced Scale Deformation in Carp (Cyprinus carpio)
复制标题
镉引起鲤鱼（Cyprinus carpio）鳞片变形
DOI：
10.1007/s001289900674
复制
发表时间：
1998
影响因子：
2.7
通讯作者：
Y. Ikeda
中科院分区：
文献类型：
--
作者：
T. Yoshitomi;J. Koyama;A. Iida;N. Okamoto;Y. Ikeda
材料和方法15尾未成熟鲤鱼，平均体重57.2±6.3g(平均±SD)。将鱼分成3组，每组5尾，每组5尾，每组5尾，放置在50 L曝气池中，在25℃下用木炭过滤器过滤，在此条件下驯化30天。以每天1%体重的速度投喂冻干颗粒饲料。对照组、低剂量组和高剂量组分别给予镉含量分别为0、250和2500微克/克的氯化镉(Cd Cl2)。2.5 H2O)，分别根据Koyama和Itazawa(1977a)。这些水平已被证明会导致组织改变，但低于鱼类的致死剂量。每隔一天，一半的水族馆水就会换成淡水。水箱中的镉浓度保持在0.1微克/毫升以下。在本研究开始时，通过手术去除固定位置的鳞片，即鱼头后面和鱼体左侧侧线上方的鳞片，以进行鳞片再生研究。镉处理15天后，从每个水箱中取出鱼，并在同一地点取再生鳞片，
MATERIALS AND METHODSFifteen immature carp, Cyprinus carpio, with an average body weight of 57.2±6.3 g (mean±SD) were used. The fish were separated into three groups of five fish and each group was maintained in a 50 L aerated tank with a charcoal filter at a temperature of 25 C. Prior to the experiment, all fish were acclimated under these conditions for 30 days. The fish were fed with freeze-dried pellets at a rate of 1% body weight per day. The control group, the low-dose group and the high-dose group were given pellets containing 0, 250, and 2500 µg/g of cadmium in the form of cadmium chloride (CdCl2. 2½H2O), respectively, according to Koyama and Itazawa (1977a). These levels have been shown to cause tissue alterations but are below the lethal dose for fish. Half of the aquarium water was replaced with fresh water every other day. The concentration of cadmium in the tanks was kept at less than 0.1 µ g/ml. At the beginning of this study, a scale at a fixed position, behind the head and above the lateral line on the left side of the fish body, was surgically removed for the scale regeneration study. After 15 days of cadmium treatment, fish were taken from each tank, and a regenerating scale was taken at the same place,