Intraperitoneal Virtual Biopsy by Fibered Optical Coherence Tomography (OCT) at Natural Orifice Transluminal Endoscopic Surgery (NOTES)
Intraperitoneal Virtual Biopsy by Fibered Optical Coherence Tomography (OCT) at Natural Orifice Transluminal Endoscopic Surgery (NOTES)
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DOI：
10.1007/s11605-009-1108-z
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发表时间：
2010-04-01
影响因子：
3.2
通讯作者：
Marescaux, Jacques
中科院分区：
文献类型：
--
作者：
Cahill, Ronan A.;Asakuma, Mitsuhiro;Marescaux, Jacques
Introduction Fibered optical coherence tomography (OCT) in conjunction with natural orifice transluminal endoscopic surgery (NOTES) could provide a facility for rapid, in situ pathological diagnosis of intraperitoneal tissues in a truly minimally invasive fashion.Materials and Methods A large porcine model was established to test this hypothesis. A standard double channel gastroscope (Olympus) was used to achieve a transgastric access to the peritoneum and initiate the pneumoperitoneum. Magnetic retraction was used to display the sigmoid colon along with its mesentery. A commercially available fibered OCT probe (NIRIS system, Imalux) was inserted via a working channel of the gastroscope and used to assess intraperitoneal tissues. Separately, OCT images of human tissue specimens ex vivo were contrasted with representative standard histopathological slides.Results Intraperitoneal OCT provided clear real-time images of both the serosal and muscularis propria mural layers as well as the submuscosal-muscularis interface. Examination of mesenteric lymph nodes (including sentinel nodes) allowed visualization of their subcapsular sinus. Comparison of representative cross-sections however failed to evince sufficient resolution for confident diagnosis.Conclusion This approach is technically feasible and, if the technology is advanced and proven accurate in human patients, could potentially be used to individualize operative extent prior to definitive resection.
