In situ neutron scattering study of structural transitions in fibrin networks under shear deformation

In situ neutron scattering study of structural transitions in fibrin networks under shear deformation
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剪切变形下纤维蛋白网络结构转变的原位中子散射研究

DOI:
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发表时间:
2011
期刊:
影响因子:
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通讯作者:
D. Pozzo
D. Pozzo
中科院分区:
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文献类型:
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作者:
K. Weigandt;L. Porcar;D. Pozzo

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小角中子散射(SANS)通过直接测量纤维蛋白凝胶对简单剪切变形的结构响应,用于破译生物聚合物网络中应变硬化的起源。使用特殊的Couette剪切池系统地探测γ = 1- 170%范围内应变值下纤维蛋白凝块的结构特性。SANS结果表明,粗纤维蛋白凝胶的应变硬化反应发生在两个不同的区域,具有不同的结构和机械签名,由中间应变软化制度分开。在低应变(γ 30%)下,第二应变硬化状态与显著的纤维排列直接相关。直接从二维拟合各向异性散射数据确定的纤维的平均直径被发现在高应变制度单调减少。结果表明,纤维蛋白凝块的非线性力学性能是在低应变下的横向熵波动减少和在较高应变值下的弯曲和拉伸之间的过渡的结果。
Small angle neutron scattering (SANS) is used to decipher the origin of the strain hardening in biopolymer networks by directly measuring the structural response of a fibrin gel to simple shear deformation. A special Couette shear cell is used to systematically probe the structural properties of a fibrin clot over strain values in the range of γ = 1–170%. The SANS results indicate that the strain hardening response of coarse fibrin gels occurs in two distinct regions having different structural and mechanical signatures that are separated by an intermediate strain softening regime. At low strains (γ 30%), a second strain-hardening regime is directly correlated to significant fiber alignment. The mean diameter of the fibers determined directly from two-dimensional fits to the anisotropic scattering data is found to decrease monotonically in the high-strain regime. The results suggest that the non-linear mechanical properties of fibrin clots are the result of a reduction of lateral entropic fluctuations at low strains and a transition between bending and stretching at higher strain values.
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