RNA-Directed Amino Acid Coupling as a Model Reaction for Primitive Coded Translation
RNA-Directed Amino Acid Coupling as a Model Reaction for Primitive Coded Translation
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DOI：
10.1002/cbic.201400029
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发表时间：
2014-04-14
期刊：
影响因子：
3.2
通讯作者：
Ohkanda, Junko
中科院分区：
文献类型：
--
作者：
Harada, Kazuo;Aoyama, Shoko;Ohkanda, Junko
立体化学理论认为，原始编码翻译最初发生在RNA世界中，通过RNA指导的氨基酸偶联。在这项研究中，我们表明，HIV达特适体RNA能够识别两个连续的精氨酸残基内的达特肽，从而证明RNA可能能够定位两个氨基酸序列特异性耦合。我们还表明，这种RNA可以作为一个模板，以加速一个单一的精氨酸残基的N-末端精氨酸残基的肽引物的耦合。这一结果可能对我们理解翻译的起源有所启示。
The stereochemical theory claims that primitive coded translation initially occurred in the RNA world by RNA-directed amino acid coupling. In this study, we show that the HIV Tat aptamer RNA is capable of recognizing two consecutive arginine residues within the Tat peptide, thus demonstrating how RNA might be able to position two amino acids for sequence-specific coupling. We also show that this RNA can act as a template to accelerate the coupling of a single arginine residue to the N-terminal arginine residue of a peptide primer. The results might have implications for our understanding of the origin of translation.