The effect of graded exercise on IL-6 release and glucose uptake in human skeletal muscle
The effect of graded exercise on IL-6 release and glucose uptake in human skeletal muscle
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DOI：
10.1113/jphysiol.2002.030437
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发表时间：
2003-01-01
影响因子：
5.5
通讯作者：
Richter, EA
中科院分区：
文献类型：
--
作者：
Helge, JW;Stallknecht, B;Richter, EA
在这项研究中，假设白细胞介素（IL）-6从人体肌肉的释放与运动强度和肌肉葡萄糖摄取有关。7名健康男性在禁食过夜的状态下进行伸膝运动，双腿各以最大功率（W-max）的25%踢45 min（引出23 +/-1%的肺最大摄氧量，V叠加（O2），（最大）），然后同时用一条腿在65%和另一条腿在85%W-max下持续35分钟（40 +/-1%的肺V叠加（O2，最大））。从股动脉和两条股静脉采集血液样本，并通过热稀释法测定血流量。大腿等离子体流（休息时大腿（-1）和25%、65%和85%W-max时分别为0.15 +/-0.01、1.4 +/-0.2、2.0 +/-0.1和2.3 +/-0.2 l min（-1））和大腿摄氧量（0.02 +/-0.01，0.27 +/-0.03，0.48 +/-0.04和0.55 +/-0.051 min（-1）大腿（-1）在休息和25%，65%和85%W-max）随着运动强度的增加而增加（P <0.05）。此外，大腿IL-6释放（静息时和25%、65%和85%W-max时大腿（-1）分别为0.4 +/-0.1、1.3 +/-0.5、1.5 +/-0.6和2.5 +/-0.7 ng min（-1））和大腿葡萄糖摄取（0.05 +/-0.01、0.3 +/-0.05、0.75 +/-0.16、1.07 +/-0.15 mmol min（-1）），静息时大腿（-1）和25%、65%和85%。W-max）随运动强度的增加而增加（P <0.05）。在运动的最后35分钟，动脉中的儿茶酚胺浓度高于（P <0.05）在休息和低强度运动。在运动过程中，大腿IL-6释放与大腿葡萄糖摄取（P <0.001）和大腿葡萄糖输送（P <0.005）呈正相关，但与大腿葡萄糖提取无关。大腿IL-6释放也与动脉血浆肾上腺素浓度呈正相关。运动前肌肉糖原浓度往往与动静脉IL-6浓度差异在休息，和运动后糖原浓度呈负相关，与IL-6释放在最后35分钟的运动。总之，本研究表明，人体肌肉中IL-6的释放与运动强度、动脉肾上腺素浓度和肌肉葡萄糖摄取呈正相关。这支持了IL-6可能与运动期间葡萄糖稳态调节有关的假设。在休息和运动后观察IL-6释放和肌糖原储存之间的关系表明，IL-6可能作为碳水化合物传感器。
In this study, the hypothesis that the release of interleukin (IL)-6 from human muscle is linked to exercise intensity and muscle glucose uptake was investigated. In the overnight fasted state, seven healthy males performed knee extension exercise, kicking with both legs, each at 25% of maximal power (W-max) for 45 min (eliciting 23 +/- 1% of pulmonary maximal oxygen uptake, V overdot (O2),(max)) and then simultaneously with one leg at 65% and the other leg at 85% W-max for 35 min (40 +/- 1% of pulmonary V overdot (O2,max)). Blood was sampled from a femoral artery and both femoral veins, and blood flow was determined by thermodilution. Thigh plasma flow (0.15 +/- 0.01, 1.4 +/- 0.2, 2.0 +/- 0.1 and 2.3 +/- 0.2 l min(-1) thigh(-1) at rest and 25 %, 65 % and 85 % W-max, respectively) and thigh oxygen uptake (0.02 +/- 0.01, 0.27 +/- 0.03, 0.48 +/- 0.04 and 0.55 +/- 0.051 min(-1) thigh(-1) at rest and 25 %, 65 % and 85 % W-max, respectively) increased with increasing exercise intensity (P < 0.05). Also, thigh IL-6 release (0.4 +/- 0.1, 1.3 +/- 0.5, 1.5 +/- 0.6 and 2.5 +/- 0.7 ng min(-1) thigh(-1) at rest and 25%, 65% and 85 % W-max, respectively) and thigh glucose uptake (0.05 +/- 0.01, 0.3 +/- 0.05, 0.75 +/- 0.16, 1.07 +/- 0.15 mmol min(-1) thigh(-1) at rest and 25 %, 65 % and 85 %. W-max, respectively) increased with increasing exercise intensity (P < 0.05). During the last 35 min of exercise, arterial catecholamine concentrations were higher (P < 0.05) than at rest and during low-intensity exercise. During exercise, thigh IL-6 release was positively related to both thigh glucose uptake (P < 0.001) and thigh glucose delivery (P < 0.005), but not to thigh glucose extraction. Thigh IL-6 release was also positively related to arterial plasma adrenaline concentration. The pre-exercise muscle glycogen concentration tended to correlate with the arteriovenous IL-6 concentration difference at rest, and the postexercise glycogen concentration was inversely correlated with IL-6 release during the final 35 min of exercise. In conclusion, the study indicates that IL-6 release from human muscle is positively related to exercise intensity, arterial adrenaline concentration and muscle glucose uptake. This supports the hypothesis that IL-6 may be linked to the regulation of glucose homeostasis during exercise. The observation of a relationship between IL-6 release and muscle glycogen store both at rest and after exercise suggests that IL-6 may act as a carbohydrate sensor.