Hantavirus-Specific IgA in Saliva and Viral Antigen in the Parotid Gland in Patients With Hemorrhagic Fever With Renal Syndrome
Hantavirus-Specific IgA in Saliva and Viral Antigen in the Parotid Gland in Patients With Hemorrhagic Fever With Renal Syndrome
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DOI：
10.1002/jmv.22040
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发表时间：
2011-05-01
影响因子：
12.7
通讯作者：
Evander, Magnus
中科院分区：
文献类型：
--
作者：
Pettersson, Lisa;Rasmuson, Johan;Evander, Magnus
汉坦病毒属包括啮齿动物传播的布尼亚病毒科的人畜共患病毒，其在欧亚大陆引起肾综合征出血热（HFRS），在美洲引起汉坦病毒心肺综合征（HCPS）。啮齿类动物的唾液中含有传染性汉坦病毒，有证据表明汉坦病毒也可以在人类唾液中传播，但人与人之间的传播很罕见。在唾液中，免疫球蛋白（IG）A是主要的免疫球蛋白类别。分泌型伊加作为上皮表面的重要第一道防线，分泌型伊加与病原体的结合可以抑制微生物粘附于粘膜细胞并中和病毒。本研究通过检测肾综合征出血热急性期患者唾液中普马拉汉坦病毒（PUUV）特异性伊加和RNA，探讨唾液伊加与唾液中病毒抗原相关性的存在和重要性。33例HFRS患者唾液中PUUV特异性伊加阳性12例（36%），PUUV RNA阳性20例（61%）。唾液中伊加抗体的存在与PUUVRNA之间存在统计学显著的负相关。在长期随访中未发现唾液中的PUUV特异性伊加，而在初始研究后28-32个月的3名患者中检测到血清中的PUUV伊加。值得注意的是，PUUV RNA和PUUV核衣壳抗原检测腮腺内的HFRS患者的死亡的内皮细胞。J. Med. Virol. 83：864-870，2011. (C)2011 Wiley-Liss，Inc.
The Hantavirus genus comprises rodent borne, zoonotic viruses of the Bunyaviridae family that cause hemorrhagic fever with renal syndrome (HFRS) in Eurasia and hantavirus cardiopulmonary syndrome (HCPS) in the Americas. Rodent saliva contains infectious hantavirus and evidence suggests that hantavirus is also shed in human saliva, but person-to-person transmission is rare. In saliva, immunoglobulin (Ig) A is the predominant immunoglobulin class. Secretory IgA serves as an important first line of defence on epithelial surfaces and the binding of secretory IgA to pathogens can inhibit adherence of microorganisms to mucosal cells and neutralize viruses. This study investigated the presence and importance of salivary IgA in relation to viral antigen in the saliva by testing Puumala hantavirus (PUUV) specific IgA, and RNA in saliva in acutely ill patients with HFRS. In saliva samples, PUUV specific IgA was detected in 12 of 33 (36%) patients with HFRS and 20 (61%) were PUUV RNA positive. There was a statistically significant inverse association between the presence of salivary IgA antibodies and PUUVRNA in the saliva. PUUV-specific IgA in saliva was not found in a long-term follow-up, while PUUV IgA in serum was detected in three patients, 28-32 months after the initial study. Notably, both PUUV RNA and PUUV nucleocapsid antigen were detected in endothelial cells within the parotid gland of a deceased patient with HFRS. J. Med. Virol. 83:864-870, 2011. (C) 2011 Wiley-Liss, Inc.