EFFECTS OF THE STEEL GENE-PRODUCT ON MOUSE PRIMORDIAL GERM-CELLS IN CULTURE
EFFECTS OF THE STEEL GENE-PRODUCT ON MOUSE PRIMORDIAL GERM-CELLS IN CULTURE
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DOI：
10.1038/352807a0
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发表时间：
1991-08-29
期刊：
影响因子：
64.8
通讯作者：
WYLIE, CC
中科院分区：
文献类型：
--
作者：
GODIN, I;DEED, R;WYLIE, CC
小鼠钢铁（sl）和显性白色斑点(W)位点的突变影响原始生殖细胞（PGC）、黑色素细胞和造血干细胞1。W基因编码酪氨酸激酶家族2,3的细胞表面受体，原癌基因c-kit。原位分析显示c-kit信使RNA在早期生殖器嵴的PGC中表达。Sl基因编码这种受体的配体，一种肽生长因子，称为干细胞因子（SCF） 5-7。SCF mRNA在小鼠早期胚胎的许多区域表达，包括这些细胞类型的迁移区域8。现在重要的是确定Sl-W相互作用在这些迁移胚胎干细胞群体发育中的作用。使用体外实验系统9，我们发现SCF增加了从野生型小鼠胚胎中分离的迁移PGC的总数和集落大小，并在STO细胞（小鼠胚胎成纤维细胞系）的辐照饲养层上培养。在没有饲养细胞的情况下，SCF使培养中PGC的初始存活率和表观运动性大大增加。但是用溴脱氧尿嘧啶标记表明SCF本身并不是PGC的有丝分裂原。体外实验中，SCF对PGC没有趋化作用。这些结果表明，体内SCF是PGC存活的必要条件。这证明了一种特定的营养因子对早期种系种群的控制。
MUTATIONS at the steel (sl) and dominant white spotting (W) loci in the mouse affect primordial germ cells (PGC), melanoblasts and haemopoietic stem cells 1. The W gene encodes a cell-surface receptor of the tyrosine kinase family 2,3, the proto-oncogene c-kit. In situ analysis has shown c-kit messenger RNA expression in PGC in the early genital ridges 4. The Sl gene encodes the ligand for this receptor, a peptide growth factor, called here stem cell factor (SCF) 5-7. SCF mRNA is expressed in many regions of the early mouse embryo, including the areas of migration of these cell types 8. It is important now to identify the role of the Sl-W interaction in the development of these migratory embryonic stem cell populations. Using an in vitro assay system 9, we show that SCF increases both the overall numbers and colony sizes of migratory PGC isolated from wild-type mouse embryos, and cultured on irradiated feeder layers of STO cells (a mouse embryonic fibroblast line). In the absence of feeder cells, SCF causes a large increase in the initial survival and apparent motility of PGC in culture. But labelling with bromodeoxyuridine shows that SCF is not, by itself, a mitogen for PGC. SCF does not exert a chemotropic effect on PGC in in vitro assays. These results suggest that SCF in vivo is an essential requirement for PGC survival. This demonstrates the control of the early germ-line population by a specific trophic factor.