Cadmium accelerates bacterial oleic acid production to promote fat accumulation in Caenorhabditis elegans
Cadmium accelerates bacterial oleic acid production to promote fat accumulation in Caenorhabditis elegans
复制标题
镉加速细菌油酸产生，促进秀丽隐杆线虫脂肪积累
DOI：
10.1016/j.jhazmat.2021.126723
复制
发表时间：
2021-07-27
影响因子：
13.6
通讯作者：
Wang, Hui
中科院分区：
文献类型：
--
作者：
Ma, Xueqi;Hou, Min;Wang, Hui
环境中的镉具有较高的膳食摄入量和较长的生物半衰期，通过直接或通过肠道微生物群损害生理功能而对健康构成严重风险。然而，环境镉对微生物和宿主系统的毒性机制尚不清楚。在这里，我们建立了三个C。elegans和E.大肠杆菌培养系统，以调查镉诱导的脂质毒性的微生物的重要作用，并描绘环境镉，细菌和宿主之间的相互作用。我们发现，只有线虫在系统中的活菌，而不是紫外线杀死的细菌或没有细菌，可以诱导脂肪积累的镉暴露，表明细菌介导的影响，环境镉对身体脂肪。镉干扰了细菌代谢产物，尤其是油酸的合成，提高了合成基因的表达，促进了细菌油酸的合成，进而促进了脂质代谢相关基因的表达和脂肪沉积。不管是什么样的栽培系统。最后，我们显示了维生素D3的潜在保护作用，其显著防止了镉或油酸诱导的脂肪储存。总之，本研究阐明了镉通过细菌代谢物诱导体内脂质积累的机制，并揭示了环境镉、微生物和宿主之间的相互作用。
Environmental cadmium, with a high dietary intake and long biological half-life, is a severe health risk by harming physiological function directly or through gut microbiota. However, the toxicity mechanisms of environmental cadmium on microbes and host systems remain unclear. Herein, we established three C. elegans and E. coli cultivated systems to investigate the vital role of microorganisms in cadmium-induced lipid toxicity and depict the interaction between environmental cadmium, bacteria, and the host. We found that only nematodes in the system with live bacteria, rather than UV-killed bacteria or no bacteria, could be induced to fat accumulation by cadmium exposure, suggesting that bacteria mediated the effect of environmental cadmium on body fat. Cadmium caused perturbation of metabolite in bacteria, most notably oleic acid, elevated the synthesis genes expression, and enhanced the bacterial oleic acid production, which further promoted the expression of lipid metabolism-related genes and fat deposition in C. elegans regardless of the cultivated system. Finally, we showed the potential protective effect of Vitamin D3 which prevented cadmium- or oleic acid-induced fat storage significantly. In conclusion, this study illustrates the mechanism underlying cadmium-induced lipid accumulation in body through bacterial metabolites and reveals the interplay between environmental cadmium, microorganisms, and the host.