Mur regulates the gene encoding the manganese transporter MntH in Brucella abortus 2308.
Mur regulates the gene encoding the manganese transporter MntH in Brucella abortus 2308.
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Mur 调节流产布鲁氏菌 2308 中编码锰转运蛋白 MntH 的基因。
DOI：
10.1128/jb.05296-11
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发表时间：
2012
影响因子：
3.2
通讯作者：
Roop2nd,RMartin
中科院分区：
文献类型：
--
作者：
Menscher,EvanA;Caswell,ClaytonC;Anderson,EricS;Roop2nd,RMartin
MntH 是布鲁氏菌中唯一发现的高亲和力锰转运蛋白。先前的研究表明，MntH 是布鲁氏菌流产 2308 对小鼠的野生型毒力所必需的（Anderson ES 等人，Infect.Immun.77:3466–3474, 2009），并表明在该菌株中，mntH 基因受到 Mur 同源物以锰响应方式的调节。在此介绍的研究中，通过引物延伸分析确定了 B. abortus 2308 的转录起始位点。 Mur和mntH启动子区域之间的特异性相互作用在电泳迁移率变动测定（EMSA）中得到证实，并且通过DNase I足迹分析在mntH启动子的-55至-24区域中鉴定了Mur结合位点。 EMSA 使用寡核苷酸（其中该区域的共有核苷酸被取代）进一步评估了 Mur 与推定 Mur 盒相互作用的特异性。这些研究不仅证实了 Mur 在流产布鲁氏菌 2308 中 mntH 表达的 Mn 响应性调节中的直接作用，而且还鉴定了负责这种调节的 mntH 上游的顺式作用元件。
MntH is the only high-affinity manganese transporter identified in Brucella. A previous study showed that MntH is required for the wild-type virulence of Brucella abortus 2308 in mice (Anderson ES, et al., Infect. Immun.77:3466–3474, 2009) and indicated that themntHgene is regulated in a manganese-responsive manner in this strain by a Mur homolog. In the study presented here, the transcriptional start site formntHin B. abortus 2308 was determined by primer extension analysis. Specific interactions between Mur and themntHpromoter region were demonstrated in an electrophoretic mobility shift assay (EMSA), and a Mur binding site was identified in the −55 to −24 region of themntHpromoter by DNase I footprint analysis. The specificity of the interaction of Mur with the putative Mur box was further evaluated by EMSA employing oligonucleotides in which the consensus nucleotides in this region were substituted. These studies not only confirm a direct role for Mur in the Mn-responsive regulation ofmntHexpression in Brucella abortus 2308 but also identify thecis-acting elements upstream ofmntHthat are responsible for this regulation.