Distribution of Polysulfide in Human Biological Fluids and Their Association with Amylase and Sperm Activities
Distribution of Polysulfide in Human Biological Fluids and Their Association with Amylase and Sperm Activities
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DOI：
10.3390/molecules24091689
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发表时间：
2019-05-01
期刊：
影响因子：
4.6
通讯作者：
Ishida, Tatsuhiro
中科院分区：
文献类型：
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作者：
Ikeda, Mayumi;Ishima, Yu;Ishida, Tatsuhiro
胞内多硫化物通过其抗氧化活性调节氧化还原平衡。然而，生物体液中是否存在多硫化物仍然是个未知数。最近，我们发展了一种多硫化物的定量分析方法，发现多硫化物存在于血浆中，并对氧化应激做出反应。在这项研究中，我们证实了其他生物体液中存在多硫化物，如精液和鼻腔分泌物。比较9例特征相同的健康志愿者体液中多硫化物的含量。此外，还研究了血浆多硫化物的昼夜节律。从鼻腔分泌物和精液中检测到的多硫化物水平分别约为400M和600M。未发现血浆多硫化物与泪液、唾液和鼻液中多硫化物水平之间的相关性。精液多硫化物与血浆多硫化物呈正相关，精液中的多硫化物几乎全部存在。有趣的是，唾液和精液多硫化物分别与唾液淀粉酶和精子活动密切相关。这些结果为不孕不育和消化系统过程等多个研究领域的科学突破提供了基础。
Intracellular polysulfide could regulate the redox balance via its anti-oxidant activity. However, the existence of polysulfide in biological fluids still remains unknown. Recently, we developed a quantitative analytical method for polysulfide and discovered that polysulfide exists in plasma and responds to oxidative stress. In this study, we confirmed the presence of polysulfide in other biological fluids, such as semen and nasal discharge. The levels of polysulfide in these biological fluids from healthy volunteers (n = 9) with identical characteristics were compared. Additionally, the circadian rhythm of plasma polysulfide was also investigated. The polysulfide levels detected from nasal discharge and seminal fluid were approximately 400 and 600 M, respectively. No correlation could be found between plasma polysulfide and the polysulfide levels of tear, saliva, and nasal discharge. On the other hand, seminal polysulfide was positively correlated with plasma polysulfide, and almost all polysulfide contained in semen was found in seminal fluid. Intriguingly, saliva and seminal polysulfide strongly correlated with salivary amylase and sperm activities, respectively. These results provide a foundation for scientific breakthroughs in various research areas like infertility and the digestive system process.