Leucine Supplementation of Drinking Water Does Not Alter Susceptibility to Diet-Induced Obesity in Mice
Leucine Supplementation of Drinking Water Does Not Alter Susceptibility to Diet-Induced Obesity in Mice
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DOI：
10.3945/jn.108.100081
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发表时间：
2009-04-01
影响因子：
4.2
通讯作者：
Lynch, Christopher J.
中科院分区：
文献类型：
--
作者：
Nairizi, Ali;She, Pengxiang;Lynch, Christopher J.
支链氨基酸（BCAA）、亮氨酸（Leu）及其调控的信号通路有改善或恶化肥胖和胰岛素敏感性的报道。因此，膳食中补充亮氨酸是否有益尚不清楚。为了解决这个问题，我们研究了在饮水中添加亮氨酸（150 mmol/L；实验1和实验2）或支链氨基酸（各109 mmol/L；实验3）对单养C57BL6/J雄性小鼠饮食性肥胖（以60 kj %脂肪饮食诱导）至少14周的影响。每周评估液体和固体食物摄入量以及体重。在过去的几周里，在不同的时间取了几份血液样本，用于血浆葡萄糖、总胆固醇或亮氨酸的测量。通过间接量热法测定代谢率，通过光束断裂测定运动活动，通过H1-NMR测定身体成分和胰岛素耐量。与对照组相比，补充剂对体重、食物摄入量、耗氧量、运动活动、身体成分、胰岛素耐量或总胆固醇没有影响。在饲喂小鼠中，与对照组相比，这种补充Leu的方法仅使血浆Leu增加了76%。另一方面，在剥夺夜间食物后，即使补充组小鼠在剥夺固体食物期间持续获得含亮氨酸的水，血浆亮氨酸在两组之间也没有差异。综上所述，这些结果并没有提供证据表明，在饮用水中补充亮氨酸或支链氨基酸可以改善小鼠饮食引起的肥胖，尽管它可能改善血糖。中华医学杂志，39(3):715-719,2009。
Branched-chain amino acids (BCAA), Leu, and the signaling pathways they regulate have been reported to either improve or worsen adiposity and insulin sensitivity. Therefore, it is unclear whether dietary supplementation of Leu would be beneficial. To help address this question, we examined the effect of adding Leu (150 mmol/L; Expt. 1 and Expt. 2) or BCAA (109 mmol/L of each; Expt. 3) to the drinking water on diet-induced obesity (induced with a 60-kJ% fat diet) in singly housed C57BL6/J male mice for at least 14 wk. Liquid and solid food intakes were evaluated weekly along with body weight. During the last few weeks, several blood samples were taken at different times for plasma glucose, total cholesterol, or Leu measurements. Metabolic rate by indirect calorimetry, locomotor activity by light beam breaking, body composition by H1-NMR, and insulin tolerance were also determined. Compared with control, supplementation did not affect body weight, food intake, oxygen consumption, locomotor activity, body composition, insulin tolerance, or total cholesterol. In fed mice, this method of Leu supplementation only increased plasma Leu by 76% when the supplemented group was compared with control. On the other hand, after overnight food deprivation, the plasma Leu did not differ between these 2 groups, even though the mice in the supplemented group had continuous access to Leu-containing water during the solid food deprivation. Taken together, the results do not provide evidence that either Leu or BCAA supplementation of drinking water ameliorates diet-induced obesity in mice, although it may improve glycemia. J. Nutr. 139: 715-719, 2009.