Chemical imaging of microfluidic flows using ATR-FTIR spectroscopy
Chemical imaging of microfluidic flows using ATR-FTIR spectroscopy
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DOI：
10.1039/b909573j
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Kazarian, Sergei G.
中科院分区：
文献类型：
--
作者：
Chan, K. L. Andrew;Gulati, Shelly;Kazarian, Sergei G.
阐明微流控流动的化学成分对于理解和优化小体积环境中的反应过程都是至关重要的。在这里，我们报告了一种基于衰减全反射(ATR)-傅里叶变换红外(FTIR)光谱成像的新型检测方法的实现，该成像使用红外焦平面阵列探测器用于微流控应用。该方法是基于倒棱镜形状的ATR晶体与基于聚二甲基硅氧烷的微流控混合装置的组合。为了证明这种方法的有效性，我们报道了不同粘度的两种液体混合的直接测量和成像，以及连续H/D同位素交换的H2O和D2O的成像和混合。这种化学特定的成像方法允许直接分析流体组成随空间位置的变化，而无需使用添加的标记或染料，并可用于研究从反应到分离的许多微流体过程。
Elucidating the chemical composition of microfluidic flows is crucial in both understanding and optimising reactive processes within small-volume environments. Herein we report the implementation of a novel detection methodology based on Attenuated Total Reflection (ATR)-Fourier Transform Infra-Red (FTIR) spectroscopic imaging using an infrared focal plane array detector for microfluidic applications. The method is based on the combination of an inverted prism-shape ATR crystal with a poly(dimethylsiloxane)-based microfluidic mixing device. To demonstrate the efficacy of this approach, we report the direct measurement and imaging of the mixing of two liquids of different viscosities and the imaging and mixing of H2O and D2O with consecutive H/D isotope exchange. This chemically specific imaging approach allows direct analysis of fluid composition as a function of spatial position without the use of added labels or dyes, and can be used to study many processes in microfluidics ranging from reactions to separations.