NIR spectroscopic determination of urine components in spot urine: preliminary investigation towards optical point-of-care test
NIR spectroscopic determination of urine components in spot urine: preliminary investigation towards optical point-of-care test
复制标题
近红外光谱法测定点尿中的尿液成分：光学即时检测的初步研究
DOI：
10.1007/s11517-019-02063-1
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发表时间：
2019
影响因子：
3.2
通讯作者：
Tanaka Shinobu
中科院分区：
文献类型：
--
作者：
Suzuki Ikuto;Ogawa Mitsuhiro;Seino Kimihiro;Nogawa Masamichi;Naito Hisashi;Yamakoshi Ken-ichi;Tanaka Shinobu
24小时尿钠排泄量是人体每日钠摄入量的重要指标，目前尚无简便的测量方法，24小时尿液采集过程复杂。然而，现场尿钠与肌酐的比值(NCR)有可能评估24小时的钠排泄，并对护理点测试有用。因此，本研究旨在实现一个近红外光谱系统来评估现场尿中的NCR：(1)我们尝试使用不到10个波长来估计Na浓度；(2)我们使用我们之前报告中的肌酐浓度来计算NCR，并验证了NCR的可预测性。选用傅立叶变换红外光谱仪范围内的10个波长，通过多元线性回归分析建立了校正模型。取健康成人尿样，加入葡萄糖粉模拟糖尿病尿样。仅使用六个波长计算了NCR，结果证实了即使无机成分不吸收近红外光，估计的Na浓度也具有很高的准确性。我们的方法能够光学地估计现场尿液中的NCR，这将对护理点检测有用。
Presently, there is no convenient method to measure 24-h urinary Na excretion, which is an important index of daily Na intake, and 24-h urine collection involves a complex process. However, the Na-to-creatinine ratio (NCR) in spot urine has the potential to evaluate 24-h Na excretion and is useful for point-of-care testing. Thus, this study aimed to realize a near-infrared spectroscopic system to assess NCR in spot urine:(1) We attempted to estimate Na concentration using fewer than 10 wavelengths;(2) we calculated NCR using creatinine concentrations from our previous report and verified the NCR predictability. A calibration model was created using multi-linear regression analysis using 10 selected wavelengths in the range of Fourier-transform infrared spectrometer. Spot urine samples were obtained from healthy adults, and glucose powder was added to them to simulate diabetic samples. NCR was calculated using only six wavelengths, and the results confirmed the high accuracy of the estimated Na concentration even though inorganic components do not absorb near-infrared light. Our method enables to optically estimate NCR in spot urine, and it will be useful for point-of-care testing.