Metabolic engineering of coenzyme Q by modification of isoprenoid side chain in plant
Metabolic engineering of coenzyme Q by modification of isoprenoid side chain in plant
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DOI：
10.1016/j.febslet.2006.01.023
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发表时间：
2006-02-06
期刊：
影响因子：
3.5
通讯作者：
Kadowaki, K
中科院分区：
文献类型：
--
作者：
Takahashi, S;Ogiyama, Y;Kadowaki, K
辅酶Q（CoQ）是呼吸链中的一种电子传递分子，也是一种脂溶性抗氧化剂，几乎存在于所有生物体中。大多数谷类作物产生辅酶Q9，其中有9个异戊二烯单位。辅酶Q10含有10个异戊二烯单位，是一种非常受欢迎的食品补充剂。在这里，我们报告的基因工程水稻生产辅酶Q10使用的基因为decaprenyl二磷酸合酶（DdsA）。尽管存在内源性辅酶Q9合成，但辅酶Q9的生产几乎完全被辅酶Q10的生产所取代。设计在线粒体上表达的DdsA增加了种子中总CoQ量的积累。(c)2006年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
Coenzyme Q (CoQ), an electron transfer molecule in the respiratory, chain and a lipid-soluble antioxidant, is present in almost all organisms. Most cereal crops produce CoQ9, which has nine isoprene units. CoQ10, with 10 isoprene units, is a very popular food supplement. Here, we report the genetic engineering of rice to produce CoQ10 using the gene for decaprenyl diphosphate synthase (DdsA). The production of CoQ9 was almost completely replaced with that of CoQ10, despite the presence of endogenous CoQ9 synthesis. DdsA designed to express at the mitochondria increased accumulation of total CoQ amount in seeds. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.