Blockade of estrogen action upregulates estrogen receptor-alpha mRNA in the fetal brain.
Blockade of estrogen action upregulates estrogen receptor-alpha mRNA in the fetal brain.
复制标题
阻断雌激素作用会上调胎儿大脑中的雌激素受体-α mRNA。
DOI：
10.1159/000208793
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发表时间：
2009
期刊：
影响因子：
2.5
通讯作者：
Wood,CharlesE
中科院分区：
文献类型：
--
作者：
Schaub,ChristineE;Wood,CharlesE
背景：妊娠晚期胎儿神经内分泌的成熟对维持胎儿的动态平衡、生长发育和分娩准备至关重要。绵羊在大脑的不同区域表达雌激素受体(ER)。目的：验证孕晚期绵羊胚胎ER-α和ER-β的表达受循环雌激素影响的假说。方法：对6只长期留置导尿管的双胎绵羊分别给予赋形剂或ER阻滞剂ICI182,780 i.c.v。(0.25μg/天)。术后6~14天处死胎儿，开始输液。大脑区域被快速分离和快速冷冻，以供以后提取mRNA和蛋白质。结果：ICI182,780处理组ER-α和ER-β表达增加，小脑和海马区ER-α表达增强。结论：羊胎脑中ER-α的表达受内源性雌激素的影响，ER-β的表达受内源性雌激素的影响。
Background:Fetal neuroendocrine maturation in late gestation is critical for maintenance of fetal homeostasis, growth, and readiness for birth. Sheep express estrogen receptors (ERs) in various brain regions. However, little is known about the regulation of ER-α and ER-β in the ovine brain prenatally.Objective:The present study was designed to test the hypothesis that the expression of ER is influenced by circulating estrogens in the late-gestation sheep fetus.Methods:Six chronically-catheterized twin fetal sheep were treated with vehicle or the ER blocker ICI 182,780 i.c.v. (0.25 μg/day). Fetuses were sacrificed 6–14 days after surgery and start of infusion. Brain regions were rapidly isolated and snap-frozen for later extraction of mRNA and protein. ER-α and ER-β mRNA was measured using real-time PCR and protein was measured using Western blot.Results:Treatment with ICI 182,780 increased ER-α mRNA, especially in cerebellum and hippocampus. There were no changes in ER-α protein and no changes in ER-β at either the mRNA or protein level.Conclusion:Expression of ER-α is influenced by endogenous estrogens in the ovine fetal brain.