Cytokine appearance in human endotoxemia and primate bacteremia.
Cytokine appearance in human endotoxemia and primate bacteremia.
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发表时间：
1988-02
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影响因子：
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通讯作者：
D. G. Hesse;K. Tracey;Y. Fong;K. Manogue;Palladino Ma;A. Cerami;G. Shires;S. Lowry
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作者：
D. G. Hesse;K. Tracey;Y. Fong;K. Manogue;Palladino Ma;A. Cerami;G. Shires;S. Lowry
最近的研究结果表明，内毒素可诱导多种免疫肽细胞因子的产生，这些细胞因子可能参与感染性休克的发生发展。4名志愿者注射内毒素(每公斤体重20个单位)后，血清cachetin/肿瘤坏死因子(TNF)水平在激发后90分钟内达到峰值358+/-166微微克/毫升(与基线相比p<0.05)，血清白介素1水平在激发后2小时内达到2.14+/-0.89单位/毫升。相比之下，将致死剂量的活大肠埃希氏菌注射到四只狒狒体内，结果显示，在注射细菌后90分钟内，血清cachectin/TNF的峰值水平为20,500+/-9,890皮克/毫升(与基线相比p<0.05)，而白介素1的峰值水平在细菌攻击后3小时内为14.2+/-10.1单位/毫升。在攻击后6小时，两种模型均未观察到可检测到的单核细胞因子水平。在注射细菌后8小时，狒狒血清中的干扰素-伽马水平达到2.67+/-1.66毫微克/毫升的峰值，12小时后不再检测到。在人类血清中未检测到干扰素-γ。这些结果表明，cachectin/TNF的瞬时释放，随后是白细胞介素1和干扰素-γ，可能参与了压倒性细菌入侵的一连串事件。
The results of recent work have demonstrated that endotoxin elicits the production of several immunopeptide cytokines that likely mediate the development of septic shock. Bolus injection of endotoxin (20 units per kilogram of body weight) to four volunteers resulted in peak serum cachetin/tumor necrosis factor (TNF) levels of 358 +/- 166 picograms per milliliter within 90 minutes after challenge (p less than 0.05 versus base line) and peak serum interleukin-1 levels of 2.14 +/- 0.89 units per milliliter within two hours after challenge. By contrast, the infusion of a lethal dose of live Escherichia coli to four baboons revealed peak serum cachectin/TNF levels of 20,500 +/- 9,890 picograms per milliliter within 90 minutes after bacteria were given (p less than 0.05 versus base line) and peak interleukin-1 levels of 14.2 +/- 10.1 units per milliliter three hours after bacterial challenge. No detectable monokine levels were observed in either model six hours after challenge. Interferon-gamma levels reached a peak of 2.67 +/- 1.66 nanograms per milliliter in baboon sera at eight hours after bacterial infusion and was no longer detectable by 12 hours. Interferon-gamma was not detected in the sera of humans. These results suggest that the transient release of cachectin/TNF, followed by interleukin-1 and interferon-gamma, may participate in the cascade of events noted in overwhelming bacterial invasion.