Essential oils from Carex meyeriana Kunth: Optimization of hydrodistillation extraction by response surface methodology and evaluation of its antioxidant and antimicrobial activities
Essential oils from Carex meyeriana Kunth: Optimization of hydrodistillation extraction by response surface methodology and evaluation of its antioxidant and antimicrobial activities
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DOI：
10.1016/j.indcrop.2018.08.041
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发表时间：
2018-11-15
影响因子：
5.9
通讯作者：
Zhou, Hong-Li
中科院分区：
文献类型：
--
作者：
Cui, Hao;Pan, Hong-Wei;Zhou, Hong-Li
据报道，植物精油具有多种生物活性。为了研究毛蕊花挥发油（CmkEos），采用响应面法优化提取工艺，研究毛蕊花挥发油的化学成分、抗氧化活性和抗菌活性。采用水蒸气蒸馏法提取荆芥挥发油，采用气相色谱-质谱联用（GC-MS）法测定其化学成分。采用Box-Behnken设计的单因素试验和响应面法对提取时间、料液比（v/w）和粒径进行优化，优化后的提取时间为9 h，料液比为43:1 (mL/g)，粒度为10目，最佳得率为0.13% （w/w）。经气相色谱-质谱联用鉴定出25个化合物，占总油的94.94%。主要成分为棕榈酸（43.06%）、亚麻酸（28.46%）和油酸（3.68%）。采用二苯吡啶酰肼基自由基（DPPH）、2,2′-氮唑（3-乙基苯并噻唑-6-磺酸）（ABTS(+)）和还原力测定法对毛蕊草精油的抗氧化活性进行了评价，结果表明毛蕊草精油具有中等的抗氧化活性，IC50/EC50分别为2.33、10.79和12.59 mg/mL。在肉汤微量稀释试验中，毛蕊草精油对金黄色葡萄球菌、枯草芽孢杆菌、短小芽孢杆菌和大肠杆菌具有较强的抑菌活性，最低抑菌浓度（MIC）分别为3.13、100、50和100 mg/mL。荆芥精油具有较强的抗氧化和抗菌活性，在食品、制药和化工等领域具有很大的应用潜力。
Essential oils from plants were reported with multiple bioactivities. To investigate the Carex meyeriana Kunth essential oils (CmkEos), the optimization of extraction by response surface methodology, chemical composition, antioxidant, and antibacterial activities of Carex meyeriana Kunth essential oils were reported. Carex meyeriana Kunth essential oils were extracted by hydrodistillation and the chemical components were determined via gas chromatography-mass spectrometry (GC-MS). Extraction time, ratio of liquid to material (v/w), and particle size were evaluated by single-factor experiments and response surface methodology based on Box-Behnken Design and which were optimized to be 9 h, 43:1 (mL/g), and 10 mesh, respectively, with the optimal yield of 0.13% (w/w). Twenty-five compounds were identified by gas chromatography-mass spectrometry, accounting for 94.94% of the total oils. The major components were palmitic acid (43.06%), linolenic acid (28.46%), and oleic acid (3.68%). The antioxidant activity of Carex meyeriana Kunth essential oils was evaluated by Diphenyl picryl hydrazinyl radical (DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(+)), and reducing power assays, and Carex meyeriana Kunth essential oils showed moderate antioxidant activity with IC50/EC50 of 2.33, 10.79, and 12.59 mg/mL, respectively. In broth microdilution assays, Carex meyeriana Kunth essential oils demonstrated strong antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Bacillus pumilus, and Escherichia colt with minimum inhibitory concentrations (MIC) of 3.13, 100, 50, and 100 mg/mL, respectively. Carex meyeriana Kunth essential oils demonstrated strong antioxidant and antimicrobial activities, and shows great application potential in food, pharmaceutical, and chemical industries.