Protective activity of hydroxytyrosol metabolites on erythrocyte oxidative-induced hemolysis.
Protective activity of hydroxytyrosol metabolites on erythrocyte oxidative-induced hemolysis.
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DOI：
10.1021/jf4016202
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发表时间：
2013-06
影响因子：
6.1
通讯作者：
F. Paiva‐Martins;Aníbal Silva;V. Almeida;M. Carvalheira;C. Serra;José Enrique Rodrígues-Borges;J. Fernandes;L. Belo;A. Santos-Silva
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文献类型：
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作者：
F. Paiva‐Martins;Aníbal Silva;V. Almeida;M. Carvalheira;C. Serra;José Enrique Rodrígues-Borges;J. Fernandes;L. Belo;A. Santos-Silva
研究了羟基酪醇主要代谢物（同质香草醇、乙酸同质香草醇、羟基酪醇3′和4′- o-葡萄糖醛酸盐和同质香草醇4′- o-葡萄糖醛酸盐）对自由基引发剂2,2′-偶氮双（2-氨基丙烷）二盐酸（AAPH）或天然自由基引发剂H2O2诱导的红细胞氧化损伤的保护作用。在AAPH存在的情况下，除同型香草醇葡糖苷外，其余化合物均表现出对红细胞溶血的保护作用，其活性顺序为：20 μM hydroxytyrosol > hydroxytyrosol glucuronides = hydroxytyrosol acetate =同型香草醇=乙酸同型香草醇> homovanillyl alcohol glucuronide。在10 μM下，羟基酪醇、羟基酪醇醋酸酯和羟基酪醇葡萄糖醛酸盐仍然可以保护血红蛋白免受氧化和形态红细胞的改变。在H2O2存在的情况下，羟基酪醇显示出明显的保护红细胞免于氧化溶血的剂量依赖性，但羟基酪醇葡萄糖醛酸盐仅显示出与使用浓度无关的有限保护作用。
The capacity of important hydroxytyrosol metabolites (homovanillyl alcohol, hydroxytyrosol acetate, homovanillyl alcohol acetate, hydroxytyrosol 3' and 4'-O-glucuronides, and homovanillyl alcohol 4'-O-glucuronide) to protect red blood cells (RBCs) from oxidative injury induced by the radical initiator 2,2'-azo-bis(2-amidinopropane) dihydrochloride (AAPH) or by the natural radical initiator H2O2 was evaluated. In the presence of AAPH, all compounds showed to protect RBCs from hemolysis in a dose-dependent manner, exccept for the homovanillyl alcohol glucuronide, with the order of activity being at 20 μM hydroxytyrosol > hydroxytyrosol glucuronides = hydroxytyrosol acetate = homovanillyl alcohol = homovanillyl acetate > homovanillyl alcohol glucuronide. At 10 μM, hydroxytyrosol, hydroxytyrosol acetate, and hydroxytyrosol glucuronides still protected hemoglobine from oxidation and from morphological RBC changes. In the presence of H2O2, hydroxytyrosol showed to significantly protect RBCs from oxidative hemolysis in a dose-dependent manner, but the hydroxytyrosol glucuronides showed only a limited protection that was independent of the concentration used.