Reactivity spectrum of 30 monoclonal antimelanoma antibodies to a panel of 28 melanoma and control cell lines.
Reactivity spectrum of 30 monoclonal antimelanoma antibodies to a panel of 28 melanoma and control cell lines.
复制标题
30 种单克隆抗黑色素瘤抗体对一组 28 种黑色素瘤和对照细胞系的反应谱。
作者：
S. Carrel;M. Schreyer;A. Schmidt;J. Mach
1981年3月在NIH举行的第一次人类黑色素瘤单克隆抗体研讨会后，来自8个实验室的30种单克隆抗体在抗体结合放射免疫分析中使用28种不同的细胞系进行了检测。该组包括12例黑色素瘤、3例神经母细胞瘤、4例胶质瘤、1例视网膜母细胞瘤、4例结肠癌、1例肺癌、1例宫颈癌、1例子宫内膜癌和1例乳腺癌。测试的30种单克隆抗体的反应性模式显示，它们中没有一种是针对严格限制于黑素瘤的抗原的，但是其中几种识别优先在黑素瘤细胞上表达的抗原结构。发现大量抗体与胶质瘤和神经母细胞瘤交叉反应。因此，它们似乎识别神经外胚层相关的分化抗原。四个单克隆抗体在我们的实验室生产的进一步研究的免疫组织学定位的黑色素瘤相关抗原的新鲜肿瘤材料。在三层生物素-抗生物素蛋白-过氧化物酶系统中，每种抗体与肿瘤细胞显示不同的染色模式，表明它们针对不同的抗原。
Thirty monoclonal antibodies from eight laboratories exchanged after the First Workshop on Monoclonal Antibodies to Human Melanoma held in March 1981 at NIH were tested in an antibody-binding radioimmunoassay using a panel of 28 different cell lines. This panel included 12 melanomas, three neuroblastomas, four gliomas, one retinoblastoma, four colon carcinomas, one lung carcinoma, one cervical carcinoma, one endometrial carcinoma, and one breast carcinoma. The reactivity pattern of the 30 monoclonal antibodies tested showed that none of them were directed against antigens strictly restricted to melanoma, but that several of them recognize antigenic structures preferentially expressed on melanoma cells. A large number of antibodies were found to crossreact with gliomas and neuroblastomas. Thus, they seem to recognize neuroectoderm associated differentiation antigens. Four monoclonal antibodies produced in our laboratory were further studied for the immunohistological localization of melanoma associated antigens on fresh tumor material. In a three-layer biotin-avidin-peroxidase system each antibody showed a different staining pattern with the tumor cells, suggesting that they were directed against different antigens.
用单克隆抗体定义的人黑色素瘤、神经胶质瘤、神经母细胞瘤和肉瘤细胞的常见抗原决定簇。
影响因子：
11.2
作者：
Seeger,RC;Rosenblatt,HM;Imai,K;Ferrone,S
通讯作者：
Ferrone,S